Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The identification of genes involved in the stomatal response to reduced atmospheric relative humidity.
AU - Xie, Xiaodong D.
AU - Wang, Yibing B.
AU - Williamson, Lisa
AU - Holroyd, Geoff H.
AU - Tagliavia, Cecilia
AU - Murchie, Erik
AU - Theobald, Julian C.
AU - Knight, Marc R.
AU - Davies, William J.
AU - Leyser, H. M. Ottoline
AU - Hetherington, Alistair M.
PY - 2006/5/9
Y1 - 2006/5/9
N2 - Stomatal pores of higher plants close in response to decreases in atmospheric relative humidity (RH). This is believed to be a mechanism that prevents the plant from losing excess water when exposed to a dry atmosphere and as such is likely to have been of evolutionary significance during the colonization of terrestrial environments by the embryophytes. We have conducted a genetic screen, based on infrared thermal imaging, to identify Arabidopsis genes involved in the stomatal response to reduced RH. Here we report the characterization of two genes, identified during this screen, which are involved in the guard cell reduced RH signaling pathway. Both genes encode proteins known to be involved in guard cell ABA signaling. OST1 encodes a protein kinase involved in ABA-mediated stomatal closure while ABA2 encodes an enzyme involved in ABA biosynthesis. These results suggest, in contrast to previously published work, that ABA plays a role in the signal transduction pathway connecting decreases in RH to reductions in stomatal aperture. The identification of OST1 as a component required in stomatal RH and ABA signal transduction supports the proposition that guard cell signaling is organized as a network in which some intracellular signaling proteins are shared among different stimuli.
AB - Stomatal pores of higher plants close in response to decreases in atmospheric relative humidity (RH). This is believed to be a mechanism that prevents the plant from losing excess water when exposed to a dry atmosphere and as such is likely to have been of evolutionary significance during the colonization of terrestrial environments by the embryophytes. We have conducted a genetic screen, based on infrared thermal imaging, to identify Arabidopsis genes involved in the stomatal response to reduced RH. Here we report the characterization of two genes, identified during this screen, which are involved in the guard cell reduced RH signaling pathway. Both genes encode proteins known to be involved in guard cell ABA signaling. OST1 encodes a protein kinase involved in ABA-mediated stomatal closure while ABA2 encodes an enzyme involved in ABA biosynthesis. These results suggest, in contrast to previously published work, that ABA plays a role in the signal transduction pathway connecting decreases in RH to reductions in stomatal aperture. The identification of OST1 as a component required in stomatal RH and ABA signal transduction supports the proposition that guard cell signaling is organized as a network in which some intracellular signaling proteins are shared among different stimuli.
KW - ABSCISIC-ACID BIOSYNTHESIS
KW - VAPOR-PRESSURE-DEFICIT
KW - ARABIDOPSIS MUTANTS
KW - PROTEIN-KINASE
KW - GUARD-CELLS
KW - AIR
KW - CO2
KW - LEAVES
U2 - 10.1016/j.cub.2006.03.028
DO - 10.1016/j.cub.2006.03.028
M3 - Journal article
VL - 16
SP - 882
EP - 887
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 9
ER -