The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.).

Lancaster Environment Centre

Text available via DOI:

https://doi.org/10.1093/jexbot/49.318.115
Final published version

Keywords

Ozone, hydrocarbons, velvet bean, Mucuna pruriens, antioxidants.

View graph of relations

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.). / Stokes, N. J.; Terry, G. T.; Hewitt, CN.
In: Journal of Experimental Botany, Vol. 49, No. 318, 01.1998, p. 115-123.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Stokes, N. J. ; Terry, G. T. ; Hewitt, CN. / The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.). In: Journal of Experimental Botany. 1998 ; Vol. 49, No. 318. pp. 115-123.

Bibtex

@article{b0c94aee5d5648ef83c3358f46b8b3ff,

title = "The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.).",

abstract = "Four-week-old velvet beans (Mucuna pruriens L.) were fumigated with ozone, isoprene and propene both singly and in combination (hydrocarbon+oxidant) in controlled-environment chambers for a 4 week period. Measurements were made of total soluble protein, ascorbate, dehydroascorbate, lipid peroxidation, and glutathione reductase activity in mature and young velvet bean leaves.Significant increases in soluble protein concentrations with respect to the controls were found in plants fumigated with propene and isoprene+ozone for young leaves, and ozone only for mature leaves. The analysis of ascorbate concentrations in young leaves showed a significant increase in total ascorbate for propene-fumigated plants a significant decrease in the level of the oxidized form (dehydroascorbate) for ozone and isoprene+ozone-treated plants, and a significant increase in the reduced form for plants fumigated with propene, propene+ozone and isoprene+ozone. The analysis of ascorbate levels in mature leaves showed no significant effect from any fumigation regime.When compared to control material the specific activity of the enzyme glutathione reductase was found to be significantly reduced in young leaves treated with propene, isoprene+ozone and ozone alone. However, in mature leaves this effect is lost and there are no significant differences with respect to the control",

keywords = "Ozone, hydrocarbons, velvet bean, Mucuna pruriens, antioxidants.",

author = "Stokes, {N. J.} and Terry, {G. T.} and CN Hewitt",

year = "1998",

month = jan,

doi = "10.1093/jexbot/49.318.115",

language = "English",

volume = "49",

pages = "115--123",

journal = "Journal of Experimental Botany",

issn = "1460-2431",

publisher = "OXFORD UNIV PRESS",

number = "318",

}

RIS

TY - JOUR

T1 - The impact of ozone, isoprene and propene on antioxidant levels in two leaf classes of velvet beans (Mucana pruriens L.).

AU - Stokes, N. J.

AU - Terry, G. T.

AU - Hewitt, CN

PY - 1998/1

Y1 - 1998/1

N2 - Four-week-old velvet beans (Mucuna pruriens L.) were fumigated with ozone, isoprene and propene both singly and in combination (hydrocarbon+oxidant) in controlled-environment chambers for a 4 week period. Measurements were made of total soluble protein, ascorbate, dehydroascorbate, lipid peroxidation, and glutathione reductase activity in mature and young velvet bean leaves.Significant increases in soluble protein concentrations with respect to the controls were found in plants fumigated with propene and isoprene+ozone for young leaves, and ozone only for mature leaves. The analysis of ascorbate concentrations in young leaves showed a significant increase in total ascorbate for propene-fumigated plants a significant decrease in the level of the oxidized form (dehydroascorbate) for ozone and isoprene+ozone-treated plants, and a significant increase in the reduced form for plants fumigated with propene, propene+ozone and isoprene+ozone. The analysis of ascorbate levels in mature leaves showed no significant effect from any fumigation regime.When compared to control material the specific activity of the enzyme glutathione reductase was found to be significantly reduced in young leaves treated with propene, isoprene+ozone and ozone alone. However, in mature leaves this effect is lost and there are no significant differences with respect to the control

AB - Four-week-old velvet beans (Mucuna pruriens L.) were fumigated with ozone, isoprene and propene both singly and in combination (hydrocarbon+oxidant) in controlled-environment chambers for a 4 week period. Measurements were made of total soluble protein, ascorbate, dehydroascorbate, lipid peroxidation, and glutathione reductase activity in mature and young velvet bean leaves.Significant increases in soluble protein concentrations with respect to the controls were found in plants fumigated with propene and isoprene+ozone for young leaves, and ozone only for mature leaves. The analysis of ascorbate concentrations in young leaves showed a significant increase in total ascorbate for propene-fumigated plants a significant decrease in the level of the oxidized form (dehydroascorbate) for ozone and isoprene+ozone-treated plants, and a significant increase in the reduced form for plants fumigated with propene, propene+ozone and isoprene+ozone. The analysis of ascorbate levels in mature leaves showed no significant effect from any fumigation regime.When compared to control material the specific activity of the enzyme glutathione reductase was found to be significantly reduced in young leaves treated with propene, isoprene+ozone and ozone alone. However, in mature leaves this effect is lost and there are no significant differences with respect to the control

KW - Ozone

KW - hydrocarbons

KW - velvet bean

KW - Mucuna pruriens

KW - antioxidants.

U2 - 10.1093/jexbot/49.318.115

DO - 10.1093/jexbot/49.318.115

M3 - Journal article

VL - 49

SP - 115

EP - 123

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 1460-2431

IS - 318

ER -

Research

Links

Text available via DOI:

Keywords