The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity

Associated organisational unit

MSI Member

Text available via DOI:

https://doi.org/10.1088/1361-6463/ac43d9
Final published version
Available under license: CC BY: Creative Commons Attribution 4.0 International License

View graph of relations

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity. / Ghimire, B.; Patenall, B.L.; Szili, E.J. et al.
In: Journal of Physics D: Applied Physics, Vol. 55, No. 12, 125207, 28.12.2021.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{9c60f961df39456c9e531ee24b754140,

title = "The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity",

abstract = "This study investigates how addition of a 2nd ground electrode in an argon plasma jet influences the production of hydrogen peroxide (H2O2) in deionised water (DIW). Briefly, plasma is ignited by purging argon gas through a quartz tube at 1 l min-1 and applying a sinusoidal voltage of 7 kV (peak-peak) at 23.5 kHz to a high voltage stainless steel needle electrode sealed inside the quartz tube surrounded by one or two copper ring(s) that served as the ground electrode(s) situated downstream of the high voltage electrode. The mechanisms of H2O2 production are investigated through the electrical and optical plasma properties and chemical analysis of the treated DIW. We discover that the addition of a 2nd ground electrode results in higher accumulation of charges on the inner wall surface of the quartz tube of the plasma jet assembly resulting in an increase in the discharge current and dissipated power. This further leads to an increase in the electron temperature that more than doubles the H2O2 production through dissociative recombination of water vapour molecules, whilst still maintaining a biological tissue tolerable gas temperature. The double ground electrode plasma jet is shown to be highly effective at reducing the growth of common wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus) in both planktonic and biofilm states whilst inducing a low level of cytotoxicity in HaCaT keratinocyte skin-like cells under certain conditions. The information provided in this study is useful in understanding the complex physicochemical processes that influence H2O2 production in plasma jets, which is needed to optimise the development of plasma sources for clinical applications. ",

keywords = "biofilm, cytotoxicity, P. aeruginosa, plasma jet, plasma medicine, S. aureus, wound treatment, Argon, Atmospheric pressure, Bacteria, Biofilms, Chemical analysis, Electric discharges, Hydrogen peroxide, Hydrogen production, Mammals, Optical emission spectroscopy, Oxidation, Plasma jets, Quartz, Antibacterial efficacy, Argon plasma jets, Deionised waters, Hydrogen peroxide production, Mammalian cells, P.aeruginosa, Plasma medicines, Quartz tubes, Wound treatment, Deionized water",

author = "B. Ghimire and B.L. Patenall and E.J. Szili and N. Gaur and P. Lamichhane and N.T. Thet and D. Trivedi and A.T.A. Jenkins and R.D. Short",

year = "2021",

month = dec,

day = "28",

doi = "10.1088/1361-6463/ac43d9",

language = "English",

volume = "55",

journal = "Journal of Physics D: Applied Physics",

issn = "0022-3727",

publisher = "IOP Publishing Ltd",

number = "12",

}

RIS

TY - JOUR

T1 - The influence of a second ground electrode on hydrogen peroxide production from an atmospheric pressure argon plasma jet and correlation to antibacterial efficacy and mammalian cell cytotoxicity

AU - Ghimire, B.

AU - Patenall, B.L.

AU - Szili, E.J.

AU - Gaur, N.

AU - Lamichhane, P.

AU - Thet, N.T.

AU - Trivedi, D.

AU - Jenkins, A.T.A.

AU - Short, R.D.

PY - 2021/12/28

Y1 - 2021/12/28

N2 - This study investigates how addition of a 2nd ground electrode in an argon plasma jet influences the production of hydrogen peroxide (H2O2) in deionised water (DIW). Briefly, plasma is ignited by purging argon gas through a quartz tube at 1 l min-1 and applying a sinusoidal voltage of 7 kV (peak-peak) at 23.5 kHz to a high voltage stainless steel needle electrode sealed inside the quartz tube surrounded by one or two copper ring(s) that served as the ground electrode(s) situated downstream of the high voltage electrode. The mechanisms of H2O2 production are investigated through the electrical and optical plasma properties and chemical analysis of the treated DIW. We discover that the addition of a 2nd ground electrode results in higher accumulation of charges on the inner wall surface of the quartz tube of the plasma jet assembly resulting in an increase in the discharge current and dissipated power. This further leads to an increase in the electron temperature that more than doubles the H2O2 production through dissociative recombination of water vapour molecules, whilst still maintaining a biological tissue tolerable gas temperature. The double ground electrode plasma jet is shown to be highly effective at reducing the growth of common wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus) in both planktonic and biofilm states whilst inducing a low level of cytotoxicity in HaCaT keratinocyte skin-like cells under certain conditions. The information provided in this study is useful in understanding the complex physicochemical processes that influence H2O2 production in plasma jets, which is needed to optimise the development of plasma sources for clinical applications.

AB - This study investigates how addition of a 2nd ground electrode in an argon plasma jet influences the production of hydrogen peroxide (H2O2) in deionised water (DIW). Briefly, plasma is ignited by purging argon gas through a quartz tube at 1 l min-1 and applying a sinusoidal voltage of 7 kV (peak-peak) at 23.5 kHz to a high voltage stainless steel needle electrode sealed inside the quartz tube surrounded by one or two copper ring(s) that served as the ground electrode(s) situated downstream of the high voltage electrode. The mechanisms of H2O2 production are investigated through the electrical and optical plasma properties and chemical analysis of the treated DIW. We discover that the addition of a 2nd ground electrode results in higher accumulation of charges on the inner wall surface of the quartz tube of the plasma jet assembly resulting in an increase in the discharge current and dissipated power. This further leads to an increase in the electron temperature that more than doubles the H2O2 production through dissociative recombination of water vapour molecules, whilst still maintaining a biological tissue tolerable gas temperature. The double ground electrode plasma jet is shown to be highly effective at reducing the growth of common wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus) in both planktonic and biofilm states whilst inducing a low level of cytotoxicity in HaCaT keratinocyte skin-like cells under certain conditions. The information provided in this study is useful in understanding the complex physicochemical processes that influence H2O2 production in plasma jets, which is needed to optimise the development of plasma sources for clinical applications.

KW - biofilm

KW - cytotoxicity

KW - P. aeruginosa

KW - plasma jet

KW - plasma medicine

KW - S. aureus

KW - wound treatment

KW - Argon

KW - Atmospheric pressure

KW - Bacteria

KW - Biofilms

KW - Chemical analysis

KW - Electric discharges

KW - Hydrogen peroxide

KW - Hydrogen production

KW - Mammals

KW - Optical emission spectroscopy

KW - Oxidation

KW - Plasma jets

KW - Quartz

KW - Antibacterial efficacy

KW - Argon plasma jets

KW - Deionised waters

KW - Hydrogen peroxide production

KW - Mammalian cells

KW - P.aeruginosa

KW - Plasma medicines

KW - Quartz tubes

KW - Wound treatment

KW - Deionized water

U2 - 10.1088/1361-6463/ac43d9

DO - 10.1088/1361-6463/ac43d9

M3 - Journal article

VL - 55

JO - Journal of Physics D: Applied Physics

JF - Journal of Physics D: Applied Physics

SN - 0022-3727

IS - 12

M1 - 125207

ER -

Research

Associated organisational unit

Links

Text available via DOI:

Keywords