Rights statement: This is the author’s version of a work that was accepted for publication in Biomaterial Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterial Advances, 133, 112632, 2022 DOI: 10.1016/j.msec.2021.112632
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The influence of Ca/Mg ratio on autogelation of hydrogel biomaterials with bioceramic compounds
AU - Abalymov, Anatoly
AU - Lengert, Ekaterina
AU - Van der Meeren, Louis
AU - Saveleva, Mariia
AU - Ivanova, Anna
AU - Douglas, Timothy
AU - Douglas, E. L.
AU - Skirtach, Andre G.
AU - Volodkin, Dmitry
AU - Parakhonskiy, Bogdan V
N1 - This is the author’s version of a work that was accepted for publication in Biomaterial Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterial Advances, 133, 112632, 2022 DOI: 10.1016/j.msec.2021.112632
PY - 2022/2/28
Y1 - 2022/2/28
N2 - Hydrogels, which are versatile three-dimensional structures containing polymers and water, are very attractive for use in biomedical fields, but they suffer from rather weak mechanical properties. In this regard, biocompatible particles can be used to enhance their mechanical properties. The possibility of loading such particles with drugs (e.g. enzymes) makes them a particularly useful component in hydrogels. In this study, micro/nanoparticles containing various ratios of Ca /Mg with sizes ranging from 1 to 8 μm were prepared and mixed with gellan gum (GG) solution to study the in-situ formation of hydrogel-particle composites. The particles provide multiple functionalities: 1) they efficiently crosslink GG to induce hydrogel formation through the release of the divalent cations (Ca /Mg ) known to bind to GG polymer chains; 2) they enhance mechanical properties of the hydrogel from 2 up to 100 kPa; 3) the samples most efficiently promoting cell growth were found to contain two types of minerals: vaterite and hydroxymagnesite, which enhanced cells proliferation and hydroxyapatite formation. The results demonstrate that such composite materials are attractive candidates for applications in bone regeneration.
AB - Hydrogels, which are versatile three-dimensional structures containing polymers and water, are very attractive for use in biomedical fields, but they suffer from rather weak mechanical properties. In this regard, biocompatible particles can be used to enhance their mechanical properties. The possibility of loading such particles with drugs (e.g. enzymes) makes them a particularly useful component in hydrogels. In this study, micro/nanoparticles containing various ratios of Ca /Mg with sizes ranging from 1 to 8 μm were prepared and mixed with gellan gum (GG) solution to study the in-situ formation of hydrogel-particle composites. The particles provide multiple functionalities: 1) they efficiently crosslink GG to induce hydrogel formation through the release of the divalent cations (Ca /Mg ) known to bind to GG polymer chains; 2) they enhance mechanical properties of the hydrogel from 2 up to 100 kPa; 3) the samples most efficiently promoting cell growth were found to contain two types of minerals: vaterite and hydroxymagnesite, which enhanced cells proliferation and hydroxyapatite formation. The results demonstrate that such composite materials are attractive candidates for applications in bone regeneration.
KW - Caclium carbonate
KW - Vaterite
KW - Hydrogel
KW - Cells
KW - Gellification
KW - Gellun gum
KW - Ossification
KW - Hydroxyapatite
U2 - 10.1016/j.msec.2021.112632
DO - 10.1016/j.msec.2021.112632
M3 - Journal article
VL - 133
JO - Materials Science and Engineering: C
JF - Materials Science and Engineering: C
SN - 0928-4931
M1 - 112632
ER -