Measurements of the directivity of musical instruments are presented as part of the study of the influence of their representation in room acoustic simulations and auralizations. Pairs of measured and averaged directivities have been used both for room simulation comparisons and as a basis for listening experiments with auralizations. Room simulation results show a clear influence of the changes in the representation directivity on the distribution of acoustical parameters in the room. The results of the listening experiments with auralizations show that some changes produced by directivity variations can be perceived by the listener. Among these changes, loudness was perceived in the highest degree, followed by reverberance and clarity.