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The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi. / Obolski, Uri; Swarthout, Todd D.; Kalizang’oma, Akuzike et al.
In: Nature Communications, Vol. 14, No. 1, 7477, 17.11.2023.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Obolski, U, Swarthout, TD, Kalizang’oma, A, Mwalukomo, TS, Chan, JM, Weight, CM, Brown, C, Cave, R, Cornick, J, Kamng’ona, AW, Msefula, J, Ercoli, G, Brown, JS, Lourenço, J, Maiden, MC, French, N, Gupta, S & Heyderman, RS 2023, 'The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi', Nature Communications, vol. 14, no. 1, 7477. https://doi.org/10.1038/s41467-023-43160-y

APA

Obolski, U., Swarthout, T. D., Kalizang’oma, A., Mwalukomo, T. S., Chan, J. M., Weight, C. M., Brown, C., Cave, R., Cornick, J., Kamng’ona, A. W., Msefula, J., Ercoli, G., Brown, J. S., Lourenço, J., Maiden, M. C., French, N., Gupta, S., & Heyderman, R. S. (2023). The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi. Nature Communications, 14(1), Article 7477. https://doi.org/10.1038/s41467-023-43160-y

Vancouver

Obolski U, Swarthout TD, Kalizang’oma A, Mwalukomo TS, Chan JM, Weight CM et al. The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi. Nature Communications. 2023 Nov 17;14(1):7477. doi: 10.1038/s41467-023-43160-y

Author

Obolski, Uri ; Swarthout, Todd D. ; Kalizang’oma, Akuzike et al. / The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi. In: Nature Communications. 2023 ; Vol. 14, No. 1.

Bibtex

@article{6e6a7634440e49fbb96c761417a172c7,
title = "The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi",
abstract = "Streptococcus pneumoniae causes substantial mortality among children under 5-years-old worldwide. Polysaccharide conjugate vaccines (PCVs) are highly effective at reducing vaccine serotype disease, but emergence of non-vaccine serotypes and persistent nasopharyngeal carriage threaten this success. We investigated the hypothesis that following vaccine, adapted pneumococcal genotypes emerge with the potential for vaccine escape. We genome sequenced 2804 penumococcal isolates, collected 4-8 years after introduction of PCV13 in Blantyre, Malawi. We developed a pipeline to cluster the pneumococcal population based on metabolic core genes into “Metabolic genotypes” (MTs). We show that S. pneumoniae population genetics are characterised by emergence of MTs with distinct virulence and antimicrobial resistance (AMR) profiles. Preliminary in vitro and murine experiments revealed that representative isolates from emerging MTs differed in growth, haemolytic, epithelial infection, and murine colonisation characteristics. Our results suggest that in the context of PCV13 introduction, pneumococcal population dynamics had shifted, a phenomenon that could further undermine vaccine control and promote spread of AMR.",
author = "Uri Obolski and Swarthout, {Todd D.} and Akuzike Kalizang{\textquoteright}oma and Mwalukomo, {Thandie S.} and Chan, {Jia Mun} and Weight, {Caroline M.} and Comfort Brown and Rory Cave and Jen Cornick and Kamng{\textquoteright}ona, {Arox Wadson} and Jacquline Msefula and Giuseppe Ercoli and Brown, {Jeremy S.} and Jos{\'e} Louren{\c c}o and Maiden, {Martin C.} and Neil French and Sunetra Gupta and Heyderman, {Robert S.}",
year = "2023",
month = nov,
day = "17",
doi = "10.1038/s41467-023-43160-y",
language = "English",
volume = "14",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi

AU - Obolski, Uri

AU - Swarthout, Todd D.

AU - Kalizang’oma, Akuzike

AU - Mwalukomo, Thandie S.

AU - Chan, Jia Mun

AU - Weight, Caroline M.

AU - Brown, Comfort

AU - Cave, Rory

AU - Cornick, Jen

AU - Kamng’ona, Arox Wadson

AU - Msefula, Jacquline

AU - Ercoli, Giuseppe

AU - Brown, Jeremy S.

AU - Lourenço, José

AU - Maiden, Martin C.

AU - French, Neil

AU - Gupta, Sunetra

AU - Heyderman, Robert S.

PY - 2023/11/17

Y1 - 2023/11/17

N2 - Streptococcus pneumoniae causes substantial mortality among children under 5-years-old worldwide. Polysaccharide conjugate vaccines (PCVs) are highly effective at reducing vaccine serotype disease, but emergence of non-vaccine serotypes and persistent nasopharyngeal carriage threaten this success. We investigated the hypothesis that following vaccine, adapted pneumococcal genotypes emerge with the potential for vaccine escape. We genome sequenced 2804 penumococcal isolates, collected 4-8 years after introduction of PCV13 in Blantyre, Malawi. We developed a pipeline to cluster the pneumococcal population based on metabolic core genes into “Metabolic genotypes” (MTs). We show that S. pneumoniae population genetics are characterised by emergence of MTs with distinct virulence and antimicrobial resistance (AMR) profiles. Preliminary in vitro and murine experiments revealed that representative isolates from emerging MTs differed in growth, haemolytic, epithelial infection, and murine colonisation characteristics. Our results suggest that in the context of PCV13 introduction, pneumococcal population dynamics had shifted, a phenomenon that could further undermine vaccine control and promote spread of AMR.

AB - Streptococcus pneumoniae causes substantial mortality among children under 5-years-old worldwide. Polysaccharide conjugate vaccines (PCVs) are highly effective at reducing vaccine serotype disease, but emergence of non-vaccine serotypes and persistent nasopharyngeal carriage threaten this success. We investigated the hypothesis that following vaccine, adapted pneumococcal genotypes emerge with the potential for vaccine escape. We genome sequenced 2804 penumococcal isolates, collected 4-8 years after introduction of PCV13 in Blantyre, Malawi. We developed a pipeline to cluster the pneumococcal population based on metabolic core genes into “Metabolic genotypes” (MTs). We show that S. pneumoniae population genetics are characterised by emergence of MTs with distinct virulence and antimicrobial resistance (AMR) profiles. Preliminary in vitro and murine experiments revealed that representative isolates from emerging MTs differed in growth, haemolytic, epithelial infection, and murine colonisation characteristics. Our results suggest that in the context of PCV13 introduction, pneumococcal population dynamics had shifted, a phenomenon that could further undermine vaccine control and promote spread of AMR.

U2 - 10.1038/s41467-023-43160-y

DO - 10.1038/s41467-023-43160-y

M3 - Journal article

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 7477

ER -