The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis

Lancaster Environment Centre

Text available via DOI:

https://doi.org/10.1007/s00425-004-1211-z
Final published version

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis. / Hoecker, Ute; Toledo-Ortiz, Gabriela; Bender, Judith et al.
In: Planta, Vol. 219, No. 2, 06.2004, p. 195-200.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{a315a084370d4599a6ec66e9c33227d5,

title = "The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis",

abstract = "Previous genetic analysis identified a component, RED1, that is required for normal de-etiolation of Arabidopsis thaliana (L.) Heynh. seedlings in continuous red light (Rc). red1 mutant seedlings exhibit elongated hypocotyls and reduced cotyledon size specifically in Rc and not in continuous far-red light (FRc). Here, we show that red1 is allelic to sur2 and atr4, and is defective in the cytochrome P450 CYP83B1, an enzyme required for normal auxin homeostasis. Two alleles of atr4, like red1, exhibit increased hypocotyl elongation and reduced cotyledon expansion in Rc but not in FRc. We further show that CYP83B1 transcript levels are elevated specifically in Rc-grown seedlings when compared with seedlings grown in darkness or FRc. Hence, the Rc-specific phenotype of the red1 mutant may indicate that Rc-induction of the CYP83B1 transcript is necessary for normal seedling de-etiolation in the wild type.",

keywords = "Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Cotyledon, Cytochrome P-450 Enzyme System, Darkness, Genes, Plant, Genetic Complementation Test, Hypocotyl, Indoleacetic Acids, Light, Models, Biological, Molecular Sequence Data, Mutation, Oxygenases, Photoreceptor Cells, Phytochrome, Phytochrome B, Seeds, Transcription Factors, Transcription, Genetic, Up-Regulation",

author = "Ute Hoecker and Gabriela Toledo-Ortiz and Judith Bender and Quail, {Peter H.}",

year = "2004",

month = jun,

doi = "10.1007/s00425-004-1211-z",

language = "English",

volume = "219",

pages = "195--200",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer",

number = "2",

}

RIS

TY - JOUR

T1 - The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis

AU - Hoecker, Ute

AU - Toledo-Ortiz, Gabriela

AU - Bender, Judith

AU - Quail, Peter H.

PY - 2004/6

Y1 - 2004/6

N2 - Previous genetic analysis identified a component, RED1, that is required for normal de-etiolation of Arabidopsis thaliana (L.) Heynh. seedlings in continuous red light (Rc). red1 mutant seedlings exhibit elongated hypocotyls and reduced cotyledon size specifically in Rc and not in continuous far-red light (FRc). Here, we show that red1 is allelic to sur2 and atr4, and is defective in the cytochrome P450 CYP83B1, an enzyme required for normal auxin homeostasis. Two alleles of atr4, like red1, exhibit increased hypocotyl elongation and reduced cotyledon expansion in Rc but not in FRc. We further show that CYP83B1 transcript levels are elevated specifically in Rc-grown seedlings when compared with seedlings grown in darkness or FRc. Hence, the Rc-specific phenotype of the red1 mutant may indicate that Rc-induction of the CYP83B1 transcript is necessary for normal seedling de-etiolation in the wild type.

AB - Previous genetic analysis identified a component, RED1, that is required for normal de-etiolation of Arabidopsis thaliana (L.) Heynh. seedlings in continuous red light (Rc). red1 mutant seedlings exhibit elongated hypocotyls and reduced cotyledon size specifically in Rc and not in continuous far-red light (FRc). Here, we show that red1 is allelic to sur2 and atr4, and is defective in the cytochrome P450 CYP83B1, an enzyme required for normal auxin homeostasis. Two alleles of atr4, like red1, exhibit increased hypocotyl elongation and reduced cotyledon expansion in Rc but not in FRc. We further show that CYP83B1 transcript levels are elevated specifically in Rc-grown seedlings when compared with seedlings grown in darkness or FRc. Hence, the Rc-specific phenotype of the red1 mutant may indicate that Rc-induction of the CYP83B1 transcript is necessary for normal seedling de-etiolation in the wild type.

KW - Amino Acid Sequence

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Cotyledon

KW - Cytochrome P-450 Enzyme System

KW - Darkness

KW - Genes, Plant

KW - Genetic Complementation Test

KW - Hypocotyl

KW - Indoleacetic Acids

KW - Light

KW - Models, Biological

KW - Molecular Sequence Data

KW - Mutation

KW - Oxygenases

KW - Photoreceptor Cells

KW - Phytochrome

KW - Phytochrome B

KW - Seeds

KW - Transcription Factors

KW - Transcription, Genetic

KW - Up-Regulation

U2 - 10.1007/s00425-004-1211-z

DO - 10.1007/s00425-004-1211-z

M3 - Journal article

C2 - 14963708

VL - 219

SP - 195

EP - 200

JO - Planta

JF - Planta

SN - 0032-0935

IS - 2

ER -

Research

Links

Text available via DOI:

Keywords