Rights statement: This is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, 205, 2021 DOI: 10.1016/j.colsurfb.2021.111848
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - The response of macrophages and their osteogenic potential modulated by micro/nano-structured Ti surfaces
AU - Liu, W.
AU - Liang, L.
AU - Liu, B.
AU - Zhao, D.
AU - Tian, Y.
AU - Huang, Q.
AU - Wu, H.
N1 - This is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, 205, 2021 DOI: 10.1016/j.colsurfb.2021.111848
PY - 2021/9/30
Y1 - 2021/9/30
N2 - Current understanding on the interactions between micro/nano-structured Ti surfaces and macrophages is still limited. In this work, TiO2 nano-structures were introduced onto acid-etched Ti surfaces by alkali-heat treatment, ion exchange and subsequent heat treatment. By adjusting the concentration of NaOH during alkali-heat treatment, nano-flakes, nano-flakes mixed with nano-wires or nano-wires could formed on acid-etched Ti surfaces. The micro- and micro/nano-structured Ti surfaces possessed similar surface chemical and phase compositions. In vitro results indicate that the morphology of macrophages was highly dependent on the morphological features of nano-structures. Nano-flakes and nano-wires were favorable to induce the formation of lamellipodia and filopodia, respectively. Compared to micro-structured Ti surface, micro/nano-structured Ti surfaces polarized macrophages to their M2 phenotype and enhanced the gene expressions of osteogenic growth factors in macrophages. The M2 polarized macrophages promoted the maturation of osteoblasts. Compared to that with nano-flakes or nano-wires, the surface with mixed features of nano-flakes and nano-wires exhibited stronger anti-inflammatory and osteo-immunomodulatory effects. The findings presented in the current work suggest that introducing micro/nano-topographies onto Ti-based implant surfaces is a promising strategy to modulate the inflammatory response and mediate osteogenesis.
AB - Current understanding on the interactions between micro/nano-structured Ti surfaces and macrophages is still limited. In this work, TiO2 nano-structures were introduced onto acid-etched Ti surfaces by alkali-heat treatment, ion exchange and subsequent heat treatment. By adjusting the concentration of NaOH during alkali-heat treatment, nano-flakes, nano-flakes mixed with nano-wires or nano-wires could formed on acid-etched Ti surfaces. The micro- and micro/nano-structured Ti surfaces possessed similar surface chemical and phase compositions. In vitro results indicate that the morphology of macrophages was highly dependent on the morphological features of nano-structures. Nano-flakes and nano-wires were favorable to induce the formation of lamellipodia and filopodia, respectively. Compared to micro-structured Ti surface, micro/nano-structured Ti surfaces polarized macrophages to their M2 phenotype and enhanced the gene expressions of osteogenic growth factors in macrophages. The M2 polarized macrophages promoted the maturation of osteoblasts. Compared to that with nano-flakes or nano-wires, the surface with mixed features of nano-flakes and nano-wires exhibited stronger anti-inflammatory and osteo-immunomodulatory effects. The findings presented in the current work suggest that introducing micro/nano-topographies onto Ti-based implant surfaces is a promising strategy to modulate the inflammatory response and mediate osteogenesis.
KW - Macrophage polarization
KW - Micro/nano-structured surface
KW - Osteoblast differentiation
KW - Surface modification
KW - Ti implant
KW - alkaline phosphatase
KW - collagen
KW - nanoflake
KW - nanomaterial
KW - nanowire
KW - sodium hydroxide
KW - titanium
KW - titanium dioxide
KW - antiinflammatory activity
KW - Article
KW - bone development
KW - cell culture
KW - cell differentiation
KW - cell growth
KW - cell interaction
KW - cell maturation
KW - cell proliferation
KW - cell structure
KW - chemical composition
KW - chemical structure
KW - concentration (parameter)
KW - controlled study
KW - enzyme activity
KW - filopodium
KW - gene expression
KW - heat
KW - human
KW - human cell
KW - immunofluorescence
KW - immunomodulation
KW - in vitro study
KW - inflammation
KW - ion exchange
KW - lamellipodium
KW - macrophage
KW - nonhuman
KW - osteoblast
KW - phenotype
KW - physical chemistry
KW - polarization
KW - priority journal
KW - protein secretion
KW - wettability
U2 - 10.1016/j.colsurfb.2021.111848
DO - 10.1016/j.colsurfb.2021.111848
M3 - Journal article
VL - 205
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
M1 - 111848
ER -