Research output: Contribution to Journal/Magazine › Journal article › peer-review
The role of TGF-βs in mammalian development and neoplasia. / Akhurst, Rosemary J.; Fitzpatrick, David R.; Fowlis, Deborah J. et al.
In: Molecular Reproduction and Development, Vol. 32, No. 2, 06.1992, p. 127-135.Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The role of TGF-βs in mammalian development and neoplasia
AU - Akhurst, Rosemary J.
AU - Fitzpatrick, David R.
AU - Fowlis, Deborah J.
AU - Gatherer, Derek
AU - Millan, Fergus A.
AU - Slager, Hans
PY - 1992/6
Y1 - 1992/6
N2 - To date, three mammalian TGF-beta isoforms have been identified, each encoded by different genetic loci. Through each is very similar in primary amino acid structure, there are clear differences both in the mature bioactive peptide region and in the latency-associated peptide, which could potentially confer differential biological specificity.As one route to investigate differential biological function in vivo we have used gene specific probes for in situ hybridization studies to examine the distribution of RNA transcripts during mammalian embryogenesis. Mouse embryos from 6 to 14.5 gestational age and human embryos from 32 to 57 days post-fertilization have been probed. A general conclusion from these studies is that each TGF-beta gene has a distinct, through overlapping, pattern of transcript distribution and that this pattern, in most cases, is conserved between mouse and man. We have focused on the biological function the TGF-betas play in certain epithelia and in cardiogenesis, which will be discussed in this presentation.
AB - To date, three mammalian TGF-beta isoforms have been identified, each encoded by different genetic loci. Through each is very similar in primary amino acid structure, there are clear differences both in the mature bioactive peptide region and in the latency-associated peptide, which could potentially confer differential biological specificity.As one route to investigate differential biological function in vivo we have used gene specific probes for in situ hybridization studies to examine the distribution of RNA transcripts during mammalian embryogenesis. Mouse embryos from 6 to 14.5 gestational age and human embryos from 32 to 57 days post-fertilization have been probed. A general conclusion from these studies is that each TGF-beta gene has a distinct, through overlapping, pattern of transcript distribution and that this pattern, in most cases, is conserved between mouse and man. We have focused on the biological function the TGF-betas play in certain epithelia and in cardiogenesis, which will be discussed in this presentation.
KW - CARDIOGENESIS
KW - EPITHELIA
KW - MOUSE DEVELOPMENT
KW - TGF-BETA
KW - TRANSFORMING GROWTH-FACTOR
KW - MOUSE EPIDERMIS
KW - EXPRESSION
KW - EMBRYO
KW - CELLS
KW - HEART
KW - RNA
KW - DIFFERENTIATION
KW - PROLIFERATION
KW - EMBRYOGENESIS
U2 - 10.1002/mrd.1080320208
DO - 10.1002/mrd.1080320208
M3 - Journal article
VL - 32
SP - 127
EP - 135
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
SN - 1040-452X
IS - 2
T2 - SYMP ON TGF-BETA AND RELATED PROTEINS IN DEVELOPMENT
Y2 - 20 September 1991 through 23 September 1991
ER -