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The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria

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The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. / Rhodes, Glenn; Bosma, Hester; Studholme, David et al.
In: Environmental Microbiology, Vol. 16, No. 8, 01.09.2014, p. 2374-2388 .

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rhodes, G, Bosma, H, Studholme, D, Arnold, D, Robert, J & Pickup, R 2014, 'The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria', Environmental Microbiology, vol. 16, no. 8, pp. 2374-2388 . https://doi.org/10.1111/1462-2920.12345

APA

Rhodes, G., Bosma, H., Studholme, D., Arnold, D., Robert, J., & Pickup, R. (2014). The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. Environmental Microbiology, 16(8), 2374-2388 . https://doi.org/10.1111/1462-2920.12345

Vancouver

Rhodes G, Bosma H, Studholme D, Arnold D, Robert J, Pickup R. The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. Environmental Microbiology. 2014 Sept 1;16(8):2374-2388 . doi: 10.1111/1462-2920.12345

Author

Rhodes, Glenn ; Bosma, Hester ; Studholme, David et al. / The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. In: Environmental Microbiology. 2014 ; Vol. 16, No. 8. pp. 2374-2388 .

Bibtex

@article{cbfe0c7c65214e289fe9272c57b0ad98,
title = "The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria",
abstract = "The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. Here, for the first time, we show that rulB on plasmid pWW0 is a hotspot for the active site-specific integration of related integron-like elements (ILEs) found in six environmental pseudomonads (strains FH1-FH6). Integration into rulB on pWW0 occurred at position 6488 generating a 3 bp direct repeat. ILEs from FH1 and FH5 were 9403 bp in length and contained eight open reading frames (ORFs), while the ILE from FH4 was 16 233 bp in length and contained 16 ORFs. In all three ILEs, the first 5.1 kb (containing ORFs 1-4) were structurally conserved and contained three predicted site-specific recombinases/integrases and a tetR homologue. Downstream of these resided ORFs of the 'variable side' with structural and sequence similarity to those encoding survival traits on the fitness enhancing plasmid pGRT1 (ILEFH1 and ILEFH5) and the NR-II virulence region of genomic island PAGI-5 (ILEFH4). Collectively, these ILEs share features with the previously described type III protein secretion system effector ILEs and are considered important to host survival and transfer of fitness enhancing and (a) virulence genes between bacteria.",
author = "Glenn Rhodes and Hester Bosma and David Studholme and Dawn Arnold and Jackson Robert and Roger Pickup",
year = "2014",
month = sep,
day = "1",
doi = "10.1111/1462-2920.12345",
language = "English",
volume = "16",
pages = "2374--2388 ",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS

TY - JOUR

T1 - The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria

AU - Rhodes, Glenn

AU - Bosma, Hester

AU - Studholme, David

AU - Arnold, Dawn

AU - Robert, Jackson

AU - Pickup, Roger

PY - 2014/9/1

Y1 - 2014/9/1

N2 - The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. Here, for the first time, we show that rulB on plasmid pWW0 is a hotspot for the active site-specific integration of related integron-like elements (ILEs) found in six environmental pseudomonads (strains FH1-FH6). Integration into rulB on pWW0 occurred at position 6488 generating a 3 bp direct repeat. ILEs from FH1 and FH5 were 9403 bp in length and contained eight open reading frames (ORFs), while the ILE from FH4 was 16 233 bp in length and contained 16 ORFs. In all three ILEs, the first 5.1 kb (containing ORFs 1-4) were structurally conserved and contained three predicted site-specific recombinases/integrases and a tetR homologue. Downstream of these resided ORFs of the 'variable side' with structural and sequence similarity to those encoding survival traits on the fitness enhancing plasmid pGRT1 (ILEFH1 and ILEFH5) and the NR-II virulence region of genomic island PAGI-5 (ILEFH4). Collectively, these ILEs share features with the previously described type III protein secretion system effector ILEs and are considered important to host survival and transfer of fitness enhancing and (a) virulence genes between bacteria.

AB - The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. Here, for the first time, we show that rulB on plasmid pWW0 is a hotspot for the active site-specific integration of related integron-like elements (ILEs) found in six environmental pseudomonads (strains FH1-FH6). Integration into rulB on pWW0 occurred at position 6488 generating a 3 bp direct repeat. ILEs from FH1 and FH5 were 9403 bp in length and contained eight open reading frames (ORFs), while the ILE from FH4 was 16 233 bp in length and contained 16 ORFs. In all three ILEs, the first 5.1 kb (containing ORFs 1-4) were structurally conserved and contained three predicted site-specific recombinases/integrases and a tetR homologue. Downstream of these resided ORFs of the 'variable side' with structural and sequence similarity to those encoding survival traits on the fitness enhancing plasmid pGRT1 (ILEFH1 and ILEFH5) and the NR-II virulence region of genomic island PAGI-5 (ILEFH4). Collectively, these ILEs share features with the previously described type III protein secretion system effector ILEs and are considered important to host survival and transfer of fitness enhancing and (a) virulence genes between bacteria.

U2 - 10.1111/1462-2920.12345

DO - 10.1111/1462-2920.12345

M3 - Journal article

VL - 16

SP - 2374

EP - 2388

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 8

ER -