Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Tight junction-related protein expression and distribution in human corneal epithelium.
AU - Ban, Yuriko
AU - Dota, Atsuyoshi
AU - Cooper, Leanne J.
AU - Fullwood, Nigel J.
AU - Nakamura, Takahiro
AU - Tsuzuki, Masakatsu
AU - Mochida, Chikako
AU - Kinoshita, Shigeru
PY - 2003/6
Y1 - 2003/6
N2 - Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.
AB - Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.
KW - tight junction
KW - corneal epithelium
KW - occludin
KW - claudin
KW - ZO-1
KW - intercellular adhesion molecules
KW - immunocytochemistry
KW - electron microscopy
U2 - 10.1016/S0014-4835(03)00054-X
DO - 10.1016/S0014-4835(03)00054-X
M3 - Journal article
VL - 76
SP - 663
EP - 669
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 1096-0007
IS - 6
ER -