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Tight junction-related protein expression and distribution in human corneal epithelium.

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Tight junction-related protein expression and distribution in human corneal epithelium. / Ban, Yuriko; Dota, Atsuyoshi; Cooper, Leanne J. et al.
In: Experimental Eye Research, Vol. 76, No. 6, 06.2003, p. 663-669.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Ban, Y, Dota, A, Cooper, LJ, Fullwood, NJ, Nakamura, T, Tsuzuki, M, Mochida, C & Kinoshita, S 2003, 'Tight junction-related protein expression and distribution in human corneal epithelium.', Experimental Eye Research, vol. 76, no. 6, pp. 663-669. https://doi.org/10.1016/S0014-4835(03)00054-X

APA

Ban, Y., Dota, A., Cooper, L. J., Fullwood, N. J., Nakamura, T., Tsuzuki, M., Mochida, C., & Kinoshita, S. (2003). Tight junction-related protein expression and distribution in human corneal epithelium. Experimental Eye Research, 76(6), 663-669. https://doi.org/10.1016/S0014-4835(03)00054-X

Vancouver

Ban Y, Dota A, Cooper LJ, Fullwood NJ, Nakamura T, Tsuzuki M et al. Tight junction-related protein expression and distribution in human corneal epithelium. Experimental Eye Research. 2003 Jun;76(6):663-669. doi: 10.1016/S0014-4835(03)00054-X

Author

Ban, Yuriko ; Dota, Atsuyoshi ; Cooper, Leanne J. et al. / Tight junction-related protein expression and distribution in human corneal epithelium. In: Experimental Eye Research. 2003 ; Vol. 76, No. 6. pp. 663-669.

Bibtex

@article{4394be5fa5484f8c89b312bb8f13c586,
title = "Tight junction-related protein expression and distribution in human corneal epithelium.",
abstract = "Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.",
keywords = "tight junction, corneal epithelium, occludin, claudin, ZO-1, intercellular adhesion molecules, immunocytochemistry, electron microscopy",
author = "Yuriko Ban and Atsuyoshi Dota and Cooper, {Leanne J.} and Fullwood, {Nigel J.} and Takahiro Nakamura and Masakatsu Tsuzuki and Chikako Mochida and Shigeru Kinoshita",
year = "2003",
month = jun,
doi = "10.1016/S0014-4835(03)00054-X",
language = "English",
volume = "76",
pages = "663--669",
journal = "Experimental Eye Research",
issn = "1096-0007",
publisher = "Academic Press Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Tight junction-related protein expression and distribution in human corneal epithelium.

AU - Ban, Yuriko

AU - Dota, Atsuyoshi

AU - Cooper, Leanne J.

AU - Fullwood, Nigel J.

AU - Nakamura, Takahiro

AU - Tsuzuki, Masakatsu

AU - Mochida, Chikako

AU - Kinoshita, Shigeru

PY - 2003/6

Y1 - 2003/6

N2 - Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.

AB - Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.

KW - tight junction

KW - corneal epithelium

KW - occludin

KW - claudin

KW - ZO-1

KW - intercellular adhesion molecules

KW - immunocytochemistry

KW - electron microscopy

U2 - 10.1016/S0014-4835(03)00054-X

DO - 10.1016/S0014-4835(03)00054-X

M3 - Journal article

VL - 76

SP - 663

EP - 669

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 1096-0007

IS - 6

ER -