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Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique

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Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. / Grau-Pujol, Berta; Martí-Soler, Helena; Escola, Valdemiro et al.
In: PLoS Neglected Tropical Diseases, Vol. 15, No. 10, e0009803, 25.10.2021.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Grau-Pujol, B, Martí-Soler, H, Escola, V, Demontis, M, Jamine, JC, Gandasegui, J, Muchisse, O, Cambra-Pelleja, M, Cossa, A, Martinez-Valladares, M, Sacoor, C, Van Lieshout, L, Cano, J, Giorgi, E & Munoz, J 2021, 'Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique', PLoS Neglected Tropical Diseases, vol. 15, no. 10, e0009803. https://doi.org/10.1371/journal.pntd.0009803

APA

Grau-Pujol, B., Martí-Soler, H., Escola, V., Demontis, M., Jamine, J. C., Gandasegui, J., Muchisse, O., Cambra-Pelleja, M., Cossa, A., Martinez-Valladares, M., Sacoor, C., Van Lieshout, L., Cano, J., Giorgi, E., & Munoz, J. (2021). Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. PLoS Neglected Tropical Diseases, 15(10), Article e0009803. https://doi.org/10.1371/journal.pntd.0009803

Vancouver

Grau-Pujol B, Martí-Soler H, Escola V, Demontis M, Jamine JC, Gandasegui J et al. Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. PLoS Neglected Tropical Diseases. 2021 Oct 25;15(10):e0009803. doi: 10.1371/journal.pntd.0009803

Author

Grau-Pujol, Berta ; Martí-Soler, Helena ; Escola, Valdemiro et al. / Towards soil-transmitted helminths transmission interruption : The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique. In: PLoS Neglected Tropical Diseases. 2021 ; Vol. 15, No. 10.

Bibtex

@article{50a5660f0d1049bd95827390ed2ee30f,
title = "Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique",
abstract = "World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%– 97%) for A. lumbricoides, 95% (CI: 88%– 98%) for T. trichiura and 95% (CI: 91%– 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate targeted interventions for transmission interruption.",
author = "Berta Grau-Pujol and Helena Mart{\'i}-Soler and Valdemiro Escola and Maria Demontis and Jamine, {Jose Carlos} and Javier Gandasegui and Osvaldo Muchisse and Maria Cambra-Pelleja and Anelsio Cossa and Maria Martinez-Valladares and Charfudin Sacoor and {Van Lieshout}, Lisette and Jorge Cano and Emanuele Giorgi and Jose Munoz",
year = "2021",
month = oct,
day = "25",
doi = "10.1371/journal.pntd.0009803",
language = "English",
volume = "15",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",
number = "10",

}

RIS

TY - JOUR

T1 - Towards soil-transmitted helminths transmission interruption

T2 - The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique

AU - Grau-Pujol, Berta

AU - Martí-Soler, Helena

AU - Escola, Valdemiro

AU - Demontis, Maria

AU - Jamine, Jose Carlos

AU - Gandasegui, Javier

AU - Muchisse, Osvaldo

AU - Cambra-Pelleja, Maria

AU - Cossa, Anelsio

AU - Martinez-Valladares, Maria

AU - Sacoor, Charfudin

AU - Van Lieshout, Lisette

AU - Cano, Jorge

AU - Giorgi, Emanuele

AU - Munoz, Jose

PY - 2021/10/25

Y1 - 2021/10/25

N2 - World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%– 97%) for A. lumbricoides, 95% (CI: 88%– 98%) for T. trichiura and 95% (CI: 91%– 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate targeted interventions for transmission interruption.

AB - World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%– 97%) for A. lumbricoides, 95% (CI: 88%– 98%) for T. trichiura and 95% (CI: 91%– 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate targeted interventions for transmission interruption.

U2 - 10.1371/journal.pntd.0009803

DO - 10.1371/journal.pntd.0009803

M3 - Journal article

VL - 15

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 10

M1 - e0009803

ER -