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    Rights statement: Copyright: 2013 Pinto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility

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Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility. / Pinto, Sofia B.; Stainton, Kirsty; Harris, Simon et al.
In: PLoS Pathogens, Vol. 9, No. 10, e1003647, 10.2013.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Pinto, SB, Stainton, K, Harris, S, Kambris, Z, Sutton, ER, Bonsall, M, Parkhill, J & Sinkins, S 2013, 'Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility', PLoS Pathogens, vol. 9, no. 10, e1003647. https://doi.org/10.1371/journal.ppat.1003647

APA

Pinto, S. B., Stainton, K., Harris, S., Kambris, Z., Sutton, E. R., Bonsall, M., Parkhill, J., & Sinkins, S. (2013). Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility. PLoS Pathogens, 9(10), Article e1003647. https://doi.org/10.1371/journal.ppat.1003647

Vancouver

Pinto SB, Stainton K, Harris S, Kambris Z, Sutton ER, Bonsall M et al. Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility. PLoS Pathogens. 2013 Oct;9(10):e1003647. doi: 10.1371/journal.ppat.1003647

Author

Pinto, Sofia B. ; Stainton, Kirsty ; Harris, Simon et al. / Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility. In: PLoS Pathogens. 2013 ; Vol. 9, No. 10.

Bibtex

@article{f1a6c90661c44f3eb692b41f6c091e6c,
title = "Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility",
abstract = "Cytoplasmic incompatibility (CI) induced by the endosymbiont Wolbachia pipientis causes complex patterns of crossing sterility between populations of the Culex pipiens group of mosquitoes. The molecular basis of the phenotype is yet to be defined. In order to investigate what host changes may underlie CI at the molecular level, we examined the transcription of a homolog of the Drosophila melanogaster gene grauzone that encodes a zinc finger protein and acts as a regulator of female meiosis, in which mutations can cause sterility. Upregulation was observed in Wolbachia-infected C. pipiens group individuals relative to Wolbachia-cured lines and the level of upregulation differed between lines that were reproductively incompatible. Knockdown analysis of this gene using RNAi showed an effect on hatch rates in a Wolbachia infected Culex molestus line. Furthermore, in later stages of development an effect on developmental progression in CI embryos occurs in bidirectionally incompatible crosses. The genome of a wPip Wolbachia strain variant from Culex molestus was sequenced and compared with the genome of a wPip variant with which it was incompatible. Three genes in inserted or deleted regions were newly identified in the C. molestus wPip genome, one of which is a transcriptional regulator labelled wtrM. When this gene was transfected into adult Culex mosquitoes, upregulation of the grauzone homolog was observed. These data suggest that Wolbachia-mediated regulation of host gene expression is a component of the mechanism of cytoplasmic incompatibility.",
author = "Pinto, {Sofia B.} and Kirsty Stainton and Simon Harris and Zakaria Kambris and Sutton, {Elizabeth R.} and Michael Bonsall and Julian Parkhill and Steven Sinkins",
note = "Copyright: 2013 Pinto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2013",
month = oct,
doi = "10.1371/journal.ppat.1003647",
language = "English",
volume = "9",
journal = "PLoS Pathogens",
issn = "1553-7374",
publisher = "Public Library of Science",
number = "10",

}

RIS

TY - JOUR

T1 - Transcriptional regulation of Culex pipiens mosquitoes by Wolbachia influences cytoplasmic incompatibility

AU - Pinto, Sofia B.

AU - Stainton, Kirsty

AU - Harris, Simon

AU - Kambris, Zakaria

AU - Sutton, Elizabeth R.

AU - Bonsall, Michael

AU - Parkhill, Julian

AU - Sinkins, Steven

N1 - Copyright: 2013 Pinto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2013/10

Y1 - 2013/10

N2 - Cytoplasmic incompatibility (CI) induced by the endosymbiont Wolbachia pipientis causes complex patterns of crossing sterility between populations of the Culex pipiens group of mosquitoes. The molecular basis of the phenotype is yet to be defined. In order to investigate what host changes may underlie CI at the molecular level, we examined the transcription of a homolog of the Drosophila melanogaster gene grauzone that encodes a zinc finger protein and acts as a regulator of female meiosis, in which mutations can cause sterility. Upregulation was observed in Wolbachia-infected C. pipiens group individuals relative to Wolbachia-cured lines and the level of upregulation differed between lines that were reproductively incompatible. Knockdown analysis of this gene using RNAi showed an effect on hatch rates in a Wolbachia infected Culex molestus line. Furthermore, in later stages of development an effect on developmental progression in CI embryos occurs in bidirectionally incompatible crosses. The genome of a wPip Wolbachia strain variant from Culex molestus was sequenced and compared with the genome of a wPip variant with which it was incompatible. Three genes in inserted or deleted regions were newly identified in the C. molestus wPip genome, one of which is a transcriptional regulator labelled wtrM. When this gene was transfected into adult Culex mosquitoes, upregulation of the grauzone homolog was observed. These data suggest that Wolbachia-mediated regulation of host gene expression is a component of the mechanism of cytoplasmic incompatibility.

AB - Cytoplasmic incompatibility (CI) induced by the endosymbiont Wolbachia pipientis causes complex patterns of crossing sterility between populations of the Culex pipiens group of mosquitoes. The molecular basis of the phenotype is yet to be defined. In order to investigate what host changes may underlie CI at the molecular level, we examined the transcription of a homolog of the Drosophila melanogaster gene grauzone that encodes a zinc finger protein and acts as a regulator of female meiosis, in which mutations can cause sterility. Upregulation was observed in Wolbachia-infected C. pipiens group individuals relative to Wolbachia-cured lines and the level of upregulation differed between lines that were reproductively incompatible. Knockdown analysis of this gene using RNAi showed an effect on hatch rates in a Wolbachia infected Culex molestus line. Furthermore, in later stages of development an effect on developmental progression in CI embryos occurs in bidirectionally incompatible crosses. The genome of a wPip Wolbachia strain variant from Culex molestus was sequenced and compared with the genome of a wPip variant with which it was incompatible. Three genes in inserted or deleted regions were newly identified in the C. molestus wPip genome, one of which is a transcriptional regulator labelled wtrM. When this gene was transfected into adult Culex mosquitoes, upregulation of the grauzone homolog was observed. These data suggest that Wolbachia-mediated regulation of host gene expression is a component of the mechanism of cytoplasmic incompatibility.

U2 - 10.1371/journal.ppat.1003647

DO - 10.1371/journal.ppat.1003647

M3 - Journal article

VL - 9

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7374

IS - 10

M1 - e1003647

ER -