Links
- https://journals.sagepub.com/doi/10.1177/0885328216650125
Final published version
Text available via DOI:
Keywords
- angiogenesis, Chitosan, cross-linked chitosan, heparin, hydrogels, Bins, Biocompatibility, Blood vessels, Chitin, Crosslinking, Polysaccharides, Pore size, Angiogenesis, Biodegradable scaffold, Chick chorioallantoic membrane, Covalently cross-linked, Cross linked chitosan, Cross-linked hydrogels, Regenerative medicine, Hydrogels, chemical compound, chitosan, molecular scaffold, polyvinyl alcohol, tolonium chloride, triethyl orthoformate, unclassified drug, cross linking reagent, formic acid derivative, protein binding, animal tissue, Article, binding affinity, biocompatibility, biodegradability, cell growth, chorioallantoic membrane assay, concentration (parameters), controlled study, covalent bond, cross linking, degradation kinetics, human, human cell, hydrogel, nonhuman, particle size, priority journal, structure analysis, thermal analysis, adsorption, animal, biodegradable implant, blood vessel, chemistry, chick embryo, chorioallantois, cytology, device failure analysis, devices, equipment design, growth, development and aging, materials testing, physiology, procedures, synthesis, tissue engineering, tissue scaffold, vascularization, Absorbable Implants, Adsorption, Animals, Blood Vessels, Chick Embryo, Chorioallantoic Membrane, Cross-Linking Reagents, Equipment Design, Equipment Failure Analysis, Formates, Heparin, Materials Testing, Neovascularization, Physiologic, Polyvinyl Alcohol, Protein Binding, Tissue Engineering, Tissue Scaffolds
Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Published
| <mark>Journal publication date</mark> | 1/06/2016 |
|---|---|
| <mark>Journal</mark> | JOURNAL OF BIOMATERIALS APPLICATIONS |
| Issue number | 4 |
| Volume | 31 |
| Number of pages | 12 |
| Pages (from-to) | 582-593 |
| Publication Status | Published |
| <mark>Original language</mark> | English |
Abstract
There is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However, these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels. In this study, we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan and polyvinyl alcohol hydrogels using triethyl orthoformate. Two concentrations of triethyl orthoformate (4 and 16%) were compared for their effects on the structure of hydrogels - their swelling, pore size, and rate of degradation and for their ability to support the growth of cells and for their heparin-binding capacity and their effects on angiogenesis in a chick chorioallantoic membrane assay. Hydrogels formed with 4 or 16% both triethyl orthoformate cross-linker were equally cyto-compatible. Hydrogels formed with 4% triethyl orthoformate absorbed slightly more water than those made with 16% triethyl orthoformate and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% triethyl orthoformate showed more blood vessel formation in the CAM assay than that formed with 4% triethyl orthoformate. © The Author(s) 2016.