Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Triethyl orthoformate mediated a novel crosslinking method for the preparation of hydrogels for tissue engineering applications
T2 - Characterization and in vitro cytocompatibility analysis
AU - Yar, M.
AU - Shahzad, S.
AU - Siddiqi, S.A.
AU - Mahmood, N.
AU - Rauf, A.
AU - Anwar, M.S.
AU - Chaudhry, A.A.
AU - Rehman, I.U.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - This paper describes the development of a new crosslinking method for the synthesis of novel hydrogel films from chitosan and PVA for potential use in various biomedical applications. These hydrogel membranes were synthesized by blending different ratios of chitosan (CS) and poly(vinyl alcohol) (PVA) solutions and were crosslinked with 2.5% (w/v) triethyl orthoformate (TEOF) in the presence of 17% (w/v) sulfuric acid. The physical/chemical interactions and the presence of specific functional groups in the synthesized materials were evaluated by Fourier transform infrared (FT-IR) spectroscopy. The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM). Thermal gravimetric analysis (TGA) proved that these crosslinked hydrogel films have good thermal stability which was decreased as the CS ratio was increased. Differential scanning calorimetry (DSC) exhibited that CS and PVA were present in the amorphous form. The solution absorption properties were performed in phosphate buffer saline (PBS) solution of pH 7.4. The 20% PVA-80% CS crosslinked hydrogel films showed a greater degree of solution absorption (183%) as compared to other compositions. The hydrogels with greater CS concentration (60% and 80%) demonstrated relatively more porous structure, better cell viability and proliferation and also revealed good blood clotting ability even after crosslinking. Based on the observed facts these hydrogels can be tailored for their potential utilization in wound healing and skin tissue engineering applications. © 2015 Elsevier B.V. All rights reserved.
AB - This paper describes the development of a new crosslinking method for the synthesis of novel hydrogel films from chitosan and PVA for potential use in various biomedical applications. These hydrogel membranes were synthesized by blending different ratios of chitosan (CS) and poly(vinyl alcohol) (PVA) solutions and were crosslinked with 2.5% (w/v) triethyl orthoformate (TEOF) in the presence of 17% (w/v) sulfuric acid. The physical/chemical interactions and the presence of specific functional groups in the synthesized materials were evaluated by Fourier transform infrared (FT-IR) spectroscopy. The morphology, structure and pore size of the materials were investigated by scanning electron microscopy (SEM). Thermal gravimetric analysis (TGA) proved that these crosslinked hydrogel films have good thermal stability which was decreased as the CS ratio was increased. Differential scanning calorimetry (DSC) exhibited that CS and PVA were present in the amorphous form. The solution absorption properties were performed in phosphate buffer saline (PBS) solution of pH 7.4. The 20% PVA-80% CS crosslinked hydrogel films showed a greater degree of solution absorption (183%) as compared to other compositions. The hydrogels with greater CS concentration (60% and 80%) demonstrated relatively more porous structure, better cell viability and proliferation and also revealed good blood clotting ability even after crosslinking. Based on the observed facts these hydrogels can be tailored for their potential utilization in wound healing and skin tissue engineering applications. © 2015 Elsevier B.V. All rights reserved.
KW - Blood coagulation
KW - Chemical crosslinking
KW - Chitosan
KW - PVA
KW - VERO cell line
KW - Wound healing
KW - Blending
KW - Blood
KW - Cell culture
KW - Characterization
KW - Chitin
KW - Crosslinking
KW - Differential scanning calorimetry
KW - Fourier transform infrared spectroscopy
KW - Functional groups
KW - Gravimetric analysis
KW - Medical applications
KW - Polyvinyl alcohols
KW - Pore size
KW - Scanning electron microscopy
KW - Thermogravimetric analysis
KW - Tissue
KW - Tissue engineering
KW - Chemical cross-linking
KW - Vero cell line
KW - Hydrogels
KW - biomaterial
KW - chitosan
KW - cross linking reagent
KW - hydrogel
KW - polyvinyl alcohol
KW - animal
KW - cell line
KW - cell proliferation
KW - cell survival
KW - chemistry
KW - Chlorocebus aethiops
KW - differential scanning calorimetry
KW - drug effects
KW - infrared spectroscopy
KW - pH
KW - porosity
KW - procedures
KW - scanning electron microscopy
KW - tissue engineering
KW - Animals
KW - Biocompatible Materials
KW - Calorimetry, Differential Scanning
KW - Cell Line
KW - Cell Proliferation
KW - Cell Survival
KW - Cercopithecus aethiops
KW - Cross-Linking Reagents
KW - Hydrogen-Ion Concentration
KW - Microscopy, Electron, Scanning
KW - Polyvinyl Alcohol
KW - Porosity
KW - Spectroscopy, Fourier Transform Infrared
KW - Tissue Engineering
KW - Vero Cells
U2 - 10.1016/j.msec.2015.06.021
DO - 10.1016/j.msec.2015.06.021
M3 - Journal article
VL - 56
SP - 154
EP - 164
JO - Materials Science and Engineering: A
JF - Materials Science and Engineering: A
SN - 0921-5093
ER -