TY - JOUR

T1 - Tri-layered functionally graded membrane for potential application in periodontal regeneration

AU - Shah, Asma Tufail

AU - Zahid, Saba

AU - Ikram, Fakhera

AU - Maqbool, Muhammad

AU - Chaudhry, Aqif Anwar

AU - Rahim, Muhammad Imran

AU - Schmidt, Franziska

AU - Goerke, Oliver

AU - Khan, Abdul Samad

AU - Rehman, Ihtesham ur

PY - 2019/10/1

Y1 - 2019/10/1

N2 - A novel tri-layered, functionally-graded chitosan membrane (FGM) with bioactive glass gradient (50%, 25%, and 0% wt.) was developed by lyophilization. A step-wise grading of chitosan, bioactive glass (BG), and Pluronic F127 was introduced into the membrane in which each layer has separate surface functions that play a role of guided tissue regeneration (GTR) membranes. The lower layer was designed to replicate alveolar bone and contains 50%wt. BG, the middle layer contains 25%wt. BG, while the upper layer was non-porous without BG and it did not support cell growth. Scanning Electron Microscopy (SEM) revealed that the lower FGM surface possessed a porous structure with embedded BG particles, while the upper surface was non-porous with interconnected architecture. The contact angle measurement confirmed that the surface with BG was hydrophilic (≈00), while the opposite surface was hydrophobic (910 ± 3.840). Both osteoblast and fibroblast cells have maximum adhesion at contact angle <80°. Alamar blue assay revealed the biocompatibility of the MC3T3-E1 mouse pre-osteoblasts cells with these membranes in vitro. The cells attachment and proliferation was seen for lower surface, while no cells adhesion was observed for the upper layer. Additionally, the interaction of the tissue with these tri-layered membranes was also investigated in vivo. Hematoxylin and eosin staining revealed the biocompatible nature of these membranes. Altogether, these results indicated that due to the biocompatible nature of these membranes, they will be a good carrier of in vivo implantation.

AB - A novel tri-layered, functionally-graded chitosan membrane (FGM) with bioactive glass gradient (50%, 25%, and 0% wt.) was developed by lyophilization. A step-wise grading of chitosan, bioactive glass (BG), and Pluronic F127 was introduced into the membrane in which each layer has separate surface functions that play a role of guided tissue regeneration (GTR) membranes. The lower layer was designed to replicate alveolar bone and contains 50%wt. BG, the middle layer contains 25%wt. BG, while the upper layer was non-porous without BG and it did not support cell growth. Scanning Electron Microscopy (SEM) revealed that the lower FGM surface possessed a porous structure with embedded BG particles, while the upper surface was non-porous with interconnected architecture. The contact angle measurement confirmed that the surface with BG was hydrophilic (≈00), while the opposite surface was hydrophobic (910 ± 3.840). Both osteoblast and fibroblast cells have maximum adhesion at contact angle <80°. Alamar blue assay revealed the biocompatibility of the MC3T3-E1 mouse pre-osteoblasts cells with these membranes in vitro. The cells attachment and proliferation was seen for lower surface, while no cells adhesion was observed for the upper layer. Additionally, the interaction of the tissue with these tri-layered membranes was also investigated in vivo. Hematoxylin and eosin staining revealed the biocompatible nature of these membranes. Altogether, these results indicated that due to the biocompatible nature of these membranes, they will be a good carrier of in vivo implantation.

KW - Animal studies

KW - Bioactivity and biocompatibility

KW - Functionally graded membrane

KW - Guided tissue regeneration

KW - MC3T3-E1

KW - Pluronic F127

U2 - 10.1016/j.msec.2019.109812

DO - 10.1016/j.msec.2019.109812

M3 - Journal article

C2 - 31349482

AN - SCOPUS:85066752734

VL - 103

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

M1 - 109812

ER -