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Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. / Muñoz, MJ; Santori, MI; Rojas, F et al.
In: Molecular and Biochemical Parasitology, Vol. 147, No. 2, 30.06.2006, p. 154-162.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Muñoz, MJ, Santori, MI, Rojas, F, Gómez, EB & Téllez-Iñón, MT 2006, 'Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant.', Molecular and Biochemical Parasitology, vol. 147, no. 2, pp. 154-162. https://doi.org/10.1016/j.molbiopara.2006.02.006

APA

Muñoz, MJ., Santori, MI., Rojas, F., Gómez, EB., & Téllez-Iñón, MT. (2006). Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. Molecular and Biochemical Parasitology, 147(2), 154-162. https://doi.org/10.1016/j.molbiopara.2006.02.006

Vancouver

Muñoz MJ, Santori MI, Rojas F, Gómez EB, Téllez-Iñón MT. Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. Molecular and Biochemical Parasitology. 2006 Jun 30;147(2):154-162. Epub 2006 Feb 28. doi: 10.1016/j.molbiopara.2006.02.006

Author

Muñoz, MJ ; Santori, MI ; Rojas, F et al. / Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. In: Molecular and Biochemical Parasitology. 2006 ; Vol. 147, No. 2. pp. 154-162.

Bibtex

@article{57a5ecf055114234ac911a8f2bde3ac6,
title = "Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant.",
abstract = "The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS–CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle.",
keywords = "Trypanosoma cruzi, CKS, CRKs, Cell cycle, Cyclins",
author = "MJ Mu{\~n}oz and MI Santori and F Rojas and EB G{\'o}mez and MT T{\'e}llez-I{\~n}{\'o}n",
year = "2006",
month = jun,
day = "30",
doi = "10.1016/j.molbiopara.2006.02.006",
language = "Undefined/Unknown",
volume = "147",
pages = "154--162",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant.

AU - Muñoz, MJ

AU - Santori, MI

AU - Rojas, F

AU - Gómez, EB

AU - Téllez-Iñón, MT

PY - 2006/6/30

Y1 - 2006/6/30

N2 - The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS–CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle.

AB - The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS–CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle.

KW - Trypanosoma cruzi

KW - CKS

KW - CRKs

KW - Cell cycle

KW - Cyclins

UR - http://europepmc.org/abstract/med/16530862

U2 - 10.1016/j.molbiopara.2006.02.006

DO - 10.1016/j.molbiopara.2006.02.006

M3 - Journal article

C2 - 16530862

VL - 147

SP - 154

EP - 162

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -