Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Molecular Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Molecular Structure, 1124, 2016 DOI: 10.1016/j.molstruc.2016.02.056
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Two-dimensional codistribution spectroscopy applied to UVRR and ROA investigations of biomolecular transitions
AU - Ramer, Georg
AU - Ashton, Lorna
N1 - This is the author’s version of a work that was accepted for publication in Journal of Molecular Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Molecular Structure, 1124, 2016 DOI: 10.1016/j.molstruc.2016.02.056
PY - 2016/11/15
Y1 - 2016/11/15
N2 - The first Raman optical activity (ROA) two-dimensional correlation spectroscopy (2DCOS) study in 2006, monitoring the temperature-induced α-helix-to-β-sheet transition in poly(l-lysine), demonstrated the versatility of 2DCOS. The combination of ROA and 2DCOS provided new ROA band assignments, enabled a direct comparison between the simultaneously collected Raman and ROA data using heterocorrelations and probed sequential information. This study also confirmed that 2DCOS can be successfully used with bisignate data, although specific care is needed when interpreting the results. However, as time has passed, doubts have been raised about not only the sequential orders reported in the study but also the general reliability of sequential data. This issue has now been addressed with the introduction of 2D codistribution (2DCDS) which is specifically designed to provide the sequence of the distributed presence of species along the perturbation variable axis. In light of these new developments in 2D correlation techniques we have revisited the original ROA data and we present our updated results. Furthermore, we demonstrate how 2DCDS can be successfully applied to bisignate data using new spectral data sets of perturbation-induced transitions in polynucleotides.
AB - The first Raman optical activity (ROA) two-dimensional correlation spectroscopy (2DCOS) study in 2006, monitoring the temperature-induced α-helix-to-β-sheet transition in poly(l-lysine), demonstrated the versatility of 2DCOS. The combination of ROA and 2DCOS provided new ROA band assignments, enabled a direct comparison between the simultaneously collected Raman and ROA data using heterocorrelations and probed sequential information. This study also confirmed that 2DCOS can be successfully used with bisignate data, although specific care is needed when interpreting the results. However, as time has passed, doubts have been raised about not only the sequential orders reported in the study but also the general reliability of sequential data. This issue has now been addressed with the introduction of 2D codistribution (2DCDS) which is specifically designed to provide the sequence of the distributed presence of species along the perturbation variable axis. In light of these new developments in 2D correlation techniques we have revisited the original ROA data and we present our updated results. Furthermore, we demonstrate how 2DCDS can be successfully applied to bisignate data using new spectral data sets of perturbation-induced transitions in polynucleotides.
KW - Two-dimensional spectroscopy
KW - Codistribution
KW - Bisignate
KW - Sequential order
KW - ROA
U2 - 10.1016/j.molstruc.2016.02.056
DO - 10.1016/j.molstruc.2016.02.056
M3 - Journal article
VL - 1124
SP - 173
EP - 179
JO - Journal of Molecular Structure
JF - Journal of Molecular Structure
SN - 0022-2860
ER -