Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Understanding glycoprotein behaviours using Raman and Raman optical activity spectroscopies
T2 - characterising the entanglement induced conformational changes in oligosaccharide chains of mucin
AU - Ashton, Lorna
AU - Pudney, P. D. A.
AU - Blanch, E. W.
AU - Yakubov, G. E.
PY - 2013/11
Y1 - 2013/11
N2 - We illustrate the great potential of Raman and ROA spectroscopies for investigating the structure and organisation of glycoproteins and the complex matrices they can form. In combination these spectroscopic techniques are sensitive to changes in conformation revealing details of secondary and tertiary structures, probing hydrogen bonding interactions, as well as resolving side chain orientation and the absolute configuration of chiral substructures. To demonstrate this potential we have characterised the structural changes in a complex glycoprotein, mucin. Spectral changes were observed during the entanglement transition as the mucin concentration was increased. By applying two-dimensional correlation analysis (2DCos) to the ROA and Raman concentration-dependent spectral sets delicate transitions in mucin conformation could also be determined. From similar to 20-40 mg/ml conformational transitions assigned mainly to the sugar N-acetyl-D-galactosamine (GalNAc), which is the linking saccharide unit to the protein backbone, were monitored. Further changes in local oligosaccharide conformation above 40 mg/ml were also monitored, together with other structural transitions observed in the protein core, particularly beta-structure formation. Consequently, these spectral techniques were shown to monitor the formation of transient entanglements formed by brush-brush interactions between oligosaccharide combs of mucin molecules identifying changes in both carbohydrate and protein moieties. This work clearly shows how these methods can be used to elucidate fresh insights into the complex behaviour of these large complex molecules. (C) 2013 Elsevier B.V. All rights reserved.
AB - We illustrate the great potential of Raman and ROA spectroscopies for investigating the structure and organisation of glycoproteins and the complex matrices they can form. In combination these spectroscopic techniques are sensitive to changes in conformation revealing details of secondary and tertiary structures, probing hydrogen bonding interactions, as well as resolving side chain orientation and the absolute configuration of chiral substructures. To demonstrate this potential we have characterised the structural changes in a complex glycoprotein, mucin. Spectral changes were observed during the entanglement transition as the mucin concentration was increased. By applying two-dimensional correlation analysis (2DCos) to the ROA and Raman concentration-dependent spectral sets delicate transitions in mucin conformation could also be determined. From similar to 20-40 mg/ml conformational transitions assigned mainly to the sugar N-acetyl-D-galactosamine (GalNAc), which is the linking saccharide unit to the protein backbone, were monitored. Further changes in local oligosaccharide conformation above 40 mg/ml were also monitored, together with other structural transitions observed in the protein core, particularly beta-structure formation. Consequently, these spectral techniques were shown to monitor the formation of transient entanglements formed by brush-brush interactions between oligosaccharide combs of mucin molecules identifying changes in both carbohydrate and protein moieties. This work clearly shows how these methods can be used to elucidate fresh insights into the complex behaviour of these large complex molecules. (C) 2013 Elsevier B.V. All rights reserved.
KW - Raman optical activity
KW - Raman spectroscopy
KW - Glycoprotein
KW - Mucin
KW - Polymer entanglement
KW - 2D correlation analysis
KW - 2-DIMENSIONAL CORRELATION SPECTROSCOPY
KW - PORCINE STOMACH MUCIN
KW - POLYPROLINE-II HELICES
KW - UNFOLDED PROTEINS
KW - RHEOLOGICAL PROPERTIES
KW - SEQUENTIAL ORDER
KW - ACTIVITY SPECTRA
KW - NUCLEIC-ACIDS
KW - GASTRIC MUCIN
KW - D-GLUCOSE
U2 - 10.1016/j.cis.2013.06.005
DO - 10.1016/j.cis.2013.06.005
M3 - Journal article
VL - 199-200
SP - 66
EP - 77
JO - Advances in Colloid and Interface Science
JF - Advances in Colloid and Interface Science
SN - 0001-8686
ER -