Rights statement: This is the peer reviewed version of the following article: Daniel Martín, Inês P. Santos, Pedro Monteiro, Joana Amaral, Ricardo M. F. Costa, Jorge Martín-Garcia, Luís A. E. Batista de Carvalho, Maria Paula M. Marques, Glória Pinto (2022), Unravelling response to Fusarium circinatum through Raman spectroscopy. Journal of Raman Spectroscopy. doi: 10.1002/jrs.6446 which has been published in final form at https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jrs.6446 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Unravelling pine response to Fusarium circinatum through Raman spectroscopy
AU - Martin, Daniel
AU - P Santos, Ines
AU - Monteiro, Pedro
AU - Amaral, Joana
AU - da Costa, Ricardo M F
AU - Martin-Garcia, Jorge
AU - Batista de Carvalho, Luis A E
AU - Marques, Maria Paula M
AU - Pinto, Gloria
N1 - This is the peer reviewed version of the following article: Daniel Martín, Inês P. Santos, Pedro Monteiro, Joana Amaral, Ricardo M. F. Costa, Jorge Martín-Garcia, Luís A. E. Batista de Carvalho, Maria Paula M. Marques, Glória Pinto (2022), Unravelling response to Fusarium circinatum through Raman spectroscopy. Journal of Raman Spectroscopy. doi: 10.1002/jrs.6446 which has been published in final form at https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jrs.6446 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2022/12/31
Y1 - 2022/12/31
N2 - Pine Pitch Canker (PPC), caused by the fungus Fusarium circinatum, is associated to significant economic and ecological losses worldwide. The effectiveness of PPC monitoring, early detection in nurseries and plantations, and the identification of resistant plant material relies on the development of objective, non-destructive and cost-effective tools. This study analysed the potential of employing Raman Spectroscopy (RS) for the early detection of biochemical changes associated with PPC in Pinus spp. with different susceptibilities to F. circinatum (highly susceptible Pinus radiata vs. relatively-resistant Pinus pinea), while unveiling possible mechanisms of action on these pathosystems. Our results indicate lignin as a key constitutive component of pine resistance against PPC and thus the potential of using this technology for the selection of PPC resistant trees. Moreover, we demonstrate the power of RS-based approaches for the rapid detection of the disease in susceptible species. Early spectral variations were found in P. radiata upon inoculation with F. circinatum from 3 days post-inoculation (dpi) onwards, whereas changes in histological analysis, relative internal stem necrosis measurements, and visual disease symptoms were only visible at 6, 8, and 9-dpi, respectively. These spectral changes have been associated to cell-wall degradation and induction of phenolic compounds synthesis upon infection in P. radiata. Altogether, we believe that RS is an innovative promising tool able to reduce disease detection time in pine species and providing an appealing alternative for the development of new and eco-friendly disease control measures.
AB - Pine Pitch Canker (PPC), caused by the fungus Fusarium circinatum, is associated to significant economic and ecological losses worldwide. The effectiveness of PPC monitoring, early detection in nurseries and plantations, and the identification of resistant plant material relies on the development of objective, non-destructive and cost-effective tools. This study analysed the potential of employing Raman Spectroscopy (RS) for the early detection of biochemical changes associated with PPC in Pinus spp. with different susceptibilities to F. circinatum (highly susceptible Pinus radiata vs. relatively-resistant Pinus pinea), while unveiling possible mechanisms of action on these pathosystems. Our results indicate lignin as a key constitutive component of pine resistance against PPC and thus the potential of using this technology for the selection of PPC resistant trees. Moreover, we demonstrate the power of RS-based approaches for the rapid detection of the disease in susceptible species. Early spectral variations were found in P. radiata upon inoculation with F. circinatum from 3 days post-inoculation (dpi) onwards, whereas changes in histological analysis, relative internal stem necrosis measurements, and visual disease symptoms were only visible at 6, 8, and 9-dpi, respectively. These spectral changes have been associated to cell-wall degradation and induction of phenolic compounds synthesis upon infection in P. radiata. Altogether, we believe that RS is an innovative promising tool able to reduce disease detection time in pine species and providing an appealing alternative for the development of new and eco-friendly disease control measures.
KW - Pine pitch canker
KW - Pinus spp., disease detection
KW - species selection
KW - plant protection
U2 - 10.1002/jrs.6446
DO - 10.1002/jrs.6446
M3 - Journal article
VL - 53
SP - 2086
EP - 2099
JO - Journal of Raman Spectroscopy
JF - Journal of Raman Spectroscopy
SN - 0377-0486
IS - 12
ER -