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Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi. / Ginger, Michael L.; Prescott, Mark C.; Reynolds, David G. et al.
In: European Journal of Biochemistry, Vol. 267, No. 9, 05.2000, p. 2555-2566.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ginger, ML, Prescott, MC, Reynolds, DG, Chance, ML & Goad, LJ 2000, 'Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi.', European Journal of Biochemistry, vol. 267, no. 9, pp. 2555-2566. https://doi.org/10.1046/j.1432-1327.2000.01261.x

APA

Ginger, M. L., Prescott, M. C., Reynolds, D. G., Chance, M. L., & Goad, L. J. (2000). Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi. European Journal of Biochemistry, 267(9), 2555-2566. https://doi.org/10.1046/j.1432-1327.2000.01261.x

Vancouver

Ginger ML, Prescott MC, Reynolds DG, Chance ML, Goad LJ. Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi. European Journal of Biochemistry. 2000 May;267(9):2555-2566. doi: 10.1046/j.1432-1327.2000.01261.x

Author

Ginger, Michael L. ; Prescott, Mark C. ; Reynolds, David G. et al. / Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi. In: European Journal of Biochemistry. 2000 ; Vol. 267, No. 9. pp. 2555-2566.

Bibtex

@article{0da349c3017d46cfaa7ecfb1c49b0af8,
title = "Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi.",
abstract = "The relative roles of acetate and leucine in the provision of a carbon source for fatty acid and sterol biosynthesis in several trypanosomatid species were investigated using 14C- and 13C-labelled acetate, glucose and leucine as substrates. Promastigotes of Leishmania species synthesized a large proportion of their sterol from leucine. L. major (LV39), L. amazonensis and L. mexicana were the most efficient utilizers of leucine, producing at least 70–77% of their sterol from leucine; L. braziliensis, L. donovani and L. tropica apparently produced less sterol from leucine (23–36%) and L. major (LV561), L. adleri and L. panamamensis were intermediate, utilizing leucine to provide 51–58% of their sterol. In all the cases the balance of the sterol produced was apparently synthesized from carbon arising from acetate. The related trypanosomatid Endotrypanum monterogeii also produced a large amount (77%) of its sterol from leucine rather than acetate. By contrast Trypanosoma cruzi elaborated only 8% of its sterol from leucine and used acetate far more effectively than the Leishmania species for sterol biosynthesis. The fatty acid moieties of the triacylglycerols and phospholipids were produced from acetate. Leucine was also incorporated into the fatty acids to varying extents in the different organisms showing that leucine can also be metabolized in trypanosomatids to generate acetyl-CoA.",
author = "Ginger, {Michael L.} and Prescott, {Mark C.} and Reynolds, {David G.} and Chance, {Michael L.} and Goad, {L. John}",
year = "2000",
month = may,
doi = "10.1046/j.1432-1327.2000.01261.x",
language = "English",
volume = "267",
pages = "2555--2566",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley-Blackwell",
number = "9",

}

RIS

TY - JOUR

T1 - Utilization of leucine and acetate as carbon sources for sterol and fatty acid biosynthesis by Old and New World Leishmania species, Endotrypanum monterogeii and Trypanosoma cruzi.

AU - Ginger, Michael L.

AU - Prescott, Mark C.

AU - Reynolds, David G.

AU - Chance, Michael L.

AU - Goad, L. John

PY - 2000/5

Y1 - 2000/5

N2 - The relative roles of acetate and leucine in the provision of a carbon source for fatty acid and sterol biosynthesis in several trypanosomatid species were investigated using 14C- and 13C-labelled acetate, glucose and leucine as substrates. Promastigotes of Leishmania species synthesized a large proportion of their sterol from leucine. L. major (LV39), L. amazonensis and L. mexicana were the most efficient utilizers of leucine, producing at least 70–77% of their sterol from leucine; L. braziliensis, L. donovani and L. tropica apparently produced less sterol from leucine (23–36%) and L. major (LV561), L. adleri and L. panamamensis were intermediate, utilizing leucine to provide 51–58% of their sterol. In all the cases the balance of the sterol produced was apparently synthesized from carbon arising from acetate. The related trypanosomatid Endotrypanum monterogeii also produced a large amount (77%) of its sterol from leucine rather than acetate. By contrast Trypanosoma cruzi elaborated only 8% of its sterol from leucine and used acetate far more effectively than the Leishmania species for sterol biosynthesis. The fatty acid moieties of the triacylglycerols and phospholipids were produced from acetate. Leucine was also incorporated into the fatty acids to varying extents in the different organisms showing that leucine can also be metabolized in trypanosomatids to generate acetyl-CoA.

AB - The relative roles of acetate and leucine in the provision of a carbon source for fatty acid and sterol biosynthesis in several trypanosomatid species were investigated using 14C- and 13C-labelled acetate, glucose and leucine as substrates. Promastigotes of Leishmania species synthesized a large proportion of their sterol from leucine. L. major (LV39), L. amazonensis and L. mexicana were the most efficient utilizers of leucine, producing at least 70–77% of their sterol from leucine; L. braziliensis, L. donovani and L. tropica apparently produced less sterol from leucine (23–36%) and L. major (LV561), L. adleri and L. panamamensis were intermediate, utilizing leucine to provide 51–58% of their sterol. In all the cases the balance of the sterol produced was apparently synthesized from carbon arising from acetate. The related trypanosomatid Endotrypanum monterogeii also produced a large amount (77%) of its sterol from leucine rather than acetate. By contrast Trypanosoma cruzi elaborated only 8% of its sterol from leucine and used acetate far more effectively than the Leishmania species for sterol biosynthesis. The fatty acid moieties of the triacylglycerols and phospholipids were produced from acetate. Leucine was also incorporated into the fatty acids to varying extents in the different organisms showing that leucine can also be metabolized in trypanosomatids to generate acetyl-CoA.

U2 - 10.1046/j.1432-1327.2000.01261.x

DO - 10.1046/j.1432-1327.2000.01261.x

M3 - Journal article

VL - 267

SP - 2555

EP - 2566

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 9

ER -