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Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).

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Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). / Rayment, Neil; Rhodes, Glenn; Hudspith, Barry et al.
In: Journal of Microbiological Methods, Vol. 224, 107001, 30.09.2024.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rayment, N, Rhodes, G, Hudspith, B, Hughes, V, Chianini, F, Agrawal, G, Bull, TJ, Pickup, R & Sanderson, J 2024, 'Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).', Journal of Microbiological Methods, vol. 224, 107001. https://doi.org/10.1016/j.mimet.2024.107001

APA

Rayment, N., Rhodes, G., Hudspith, B., Hughes, V., Chianini, F., Agrawal, G., Bull, T. J., Pickup, R., & Sanderson, J. (2024). Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). Journal of Microbiological Methods, 224, Article 107001. https://doi.org/10.1016/j.mimet.2024.107001

Vancouver

Rayment N, Rhodes G, Hudspith B, Hughes V, Chianini F, Agrawal G et al. Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). Journal of Microbiological Methods. 2024 Sept 30;224:107001. Epub 2024 Jul 20. doi: 10.1016/j.mimet.2024.107001

Author

Rayment, Neil ; Rhodes, Glenn ; Hudspith, Barry et al. / Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). In: Journal of Microbiological Methods. 2024 ; Vol. 224.

Bibtex

@article{a236368d915f464cac9a36363d527fdc,
title = "Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).",
abstract = "We describe the development, testing and specificity of a modified oligonucleotide probe for the specific detection of Mycobacterium avium subsp. paratuberculosis (MAP) in culture and in infected tissue using fluorescent in situ hybridisation and confocal microscopy. The detection of MAP in both animal and human tissue using our modified probe allows for a more rapid diagnosis of MAP infection compared to the more often applied detection methods of culture and PCR and has the potential for quantification of cellular abundance. This approach would enable earlier treatment intervention and therefore the potential for reduced morbidity.",
author = "Neil Rayment and Glenn Rhodes and Barry Hudspith and Valerie Hughes and Francesca Chianini and Gaurav Agrawal and Bull, {Tim J} and Roger Pickup and Jeremy Sanderson",
year = "2024",
month = sep,
day = "30",
doi = "10.1016/j.mimet.2024.107001",
language = "English",
volume = "224",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).

AU - Rayment, Neil

AU - Rhodes, Glenn

AU - Hudspith, Barry

AU - Hughes, Valerie

AU - Chianini, Francesca

AU - Agrawal, Gaurav

AU - Bull, Tim J

AU - Pickup, Roger

AU - Sanderson, Jeremy

PY - 2024/9/30

Y1 - 2024/9/30

N2 - We describe the development, testing and specificity of a modified oligonucleotide probe for the specific detection of Mycobacterium avium subsp. paratuberculosis (MAP) in culture and in infected tissue using fluorescent in situ hybridisation and confocal microscopy. The detection of MAP in both animal and human tissue using our modified probe allows for a more rapid diagnosis of MAP infection compared to the more often applied detection methods of culture and PCR and has the potential for quantification of cellular abundance. This approach would enable earlier treatment intervention and therefore the potential for reduced morbidity.

AB - We describe the development, testing and specificity of a modified oligonucleotide probe for the specific detection of Mycobacterium avium subsp. paratuberculosis (MAP) in culture and in infected tissue using fluorescent in situ hybridisation and confocal microscopy. The detection of MAP in both animal and human tissue using our modified probe allows for a more rapid diagnosis of MAP infection compared to the more often applied detection methods of culture and PCR and has the potential for quantification of cellular abundance. This approach would enable earlier treatment intervention and therefore the potential for reduced morbidity.

U2 - 10.1016/j.mimet.2024.107001

DO - 10.1016/j.mimet.2024.107001

M3 - Journal article

VL - 224

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

M1 - 107001

ER -