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Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills

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Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. / Zhang, Dayi; He, Yi; Wang, Yun; Wang, Hui; Wu, Lin; Aries, Eric; Huang, Wei E.

In: Microbial Biotechnology, Vol. 5, No. 1, 01.2012, p. 87-97.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Zhang, D, He, Y, Wang, Y, Wang, H, Wu, L, Aries, E & Huang, WE 2012, 'Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills', Microbial Biotechnology, vol. 5, no. 1, pp. 87-97. https://doi.org/10.1111/j.1751-7915.2011.00301.x

APA

Zhang, D., He, Y., Wang, Y., Wang, H., Wu, L., Aries, E., & Huang, W. E. (2012). Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microbial Biotechnology, 5(1), 87-97. https://doi.org/10.1111/j.1751-7915.2011.00301.x

Vancouver

Author

Zhang, Dayi ; He, Yi ; Wang, Yun ; Wang, Hui ; Wu, Lin ; Aries, Eric ; Huang, Wei E. / Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. In: Microbial Biotechnology. 2012 ; Vol. 5, No. 1. pp. 87-97.

Bibtex

@article{ceda3dac49674a2486a6a023db65fda4,
title = "Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills",
abstract = "Acinetobacter baylyi ADP1 was found to tolerate seawater and have a special ability of adhering to an oilwater interface of 1080 mu m emulsified mineral and crude oil droplets. These properties make ADP1 an ideal bacterial chassis for constructing bioreporters that are able to actively search and sense oil spill in water and soils. Acinetobacter baylyi bioreporter ADPWH_alk was developed and applied to the detection of alkanes and alkenes in water, seawater and soils. Bioreporter ADPWH_alk was able to detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. So far, ADPWH_alk is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. This bioreporter responded to the alkanes in about 30 min and it was independent to the cell growth phase because of two point mutations in alkM promoter recognized by alkane regulatory protein ALKR. ADPWH_alk was applied to detect mineral oil, Brent, Chestnut and Sirri crude oils in water and seawater in the range 0.1100 mg l-1, showing that the bioreporter oil detection was semi-quantitative. This study demonstrates that ADPWH_alk is a rapid, sensitive and semi-quantitative bioreporter that can be useful for environmental monitoring and assessment of oil spills in seawater and soils.",
keywords = "ESCHERICHIA-COLI, ACINETOBACTER SP, CRUDE-OIL, PROMOTER, HYDROXYLASE, DEGRADATION, MIDDLE-CHAIN ALKANES, TRANSCRIPTIONAL REGULATORS, SP. STRAIN ADP1, N-ALKANES",
author = "Dayi Zhang and Yi He and Yun Wang and Hui Wang and Lin Wu and Eric Aries and Huang, {Wei E.}",
year = "2012",
month = jan,
doi = "10.1111/j.1751-7915.2011.00301.x",
language = "English",
volume = "5",
pages = "87--97",
journal = "Microbial Biotechnology",
issn = "1751-7907",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills

AU - Zhang, Dayi

AU - He, Yi

AU - Wang, Yun

AU - Wang, Hui

AU - Wu, Lin

AU - Aries, Eric

AU - Huang, Wei E.

PY - 2012/1

Y1 - 2012/1

N2 - Acinetobacter baylyi ADP1 was found to tolerate seawater and have a special ability of adhering to an oilwater interface of 1080 mu m emulsified mineral and crude oil droplets. These properties make ADP1 an ideal bacterial chassis for constructing bioreporters that are able to actively search and sense oil spill in water and soils. Acinetobacter baylyi bioreporter ADPWH_alk was developed and applied to the detection of alkanes and alkenes in water, seawater and soils. Bioreporter ADPWH_alk was able to detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. So far, ADPWH_alk is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. This bioreporter responded to the alkanes in about 30 min and it was independent to the cell growth phase because of two point mutations in alkM promoter recognized by alkane regulatory protein ALKR. ADPWH_alk was applied to detect mineral oil, Brent, Chestnut and Sirri crude oils in water and seawater in the range 0.1100 mg l-1, showing that the bioreporter oil detection was semi-quantitative. This study demonstrates that ADPWH_alk is a rapid, sensitive and semi-quantitative bioreporter that can be useful for environmental monitoring and assessment of oil spills in seawater and soils.

AB - Acinetobacter baylyi ADP1 was found to tolerate seawater and have a special ability of adhering to an oilwater interface of 1080 mu m emulsified mineral and crude oil droplets. These properties make ADP1 an ideal bacterial chassis for constructing bioreporters that are able to actively search and sense oil spill in water and soils. Acinetobacter baylyi bioreporter ADPWH_alk was developed and applied to the detection of alkanes and alkenes in water, seawater and soils. Bioreporter ADPWH_alk was able to detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. So far, ADPWH_alk is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. This bioreporter responded to the alkanes in about 30 min and it was independent to the cell growth phase because of two point mutations in alkM promoter recognized by alkane regulatory protein ALKR. ADPWH_alk was applied to detect mineral oil, Brent, Chestnut and Sirri crude oils in water and seawater in the range 0.1100 mg l-1, showing that the bioreporter oil detection was semi-quantitative. This study demonstrates that ADPWH_alk is a rapid, sensitive and semi-quantitative bioreporter that can be useful for environmental monitoring and assessment of oil spills in seawater and soils.

KW - ESCHERICHIA-COLI

KW - ACINETOBACTER SP

KW - CRUDE-OIL

KW - PROMOTER

KW - HYDROXYLASE

KW - DEGRADATION

KW - MIDDLE-CHAIN ALKANES

KW - TRANSCRIPTIONAL REGULATORS

KW - SP. STRAIN ADP1

KW - N-ALKANES

U2 - 10.1111/j.1751-7915.2011.00301.x

DO - 10.1111/j.1751-7915.2011.00301.x

M3 - Journal article

VL - 5

SP - 87

EP - 97

JO - Microbial Biotechnology

JF - Microbial Biotechnology

SN - 1751-7907

IS - 1

ER -