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Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS

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Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS. / Nasaruddin, Muhammad Luqman; Holscher, Christian; Kehoe, Patrick ; Graham, Stewart F.; Green, Brian.

In: American Journal of Translational Research, Vol. 8, No. 1, 30.01.2016, p. 154-165.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Nasaruddin, ML, Holscher, C, Kehoe, P, Graham, SF & Green, B 2016, 'Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS', American Journal of Translational Research, vol. 8, no. 1, pp. 154-165. <http://www.ajtr.org/files/ajtr0018204.pdf>

APA

Nasaruddin, M. L., Holscher, C., Kehoe, P., Graham, S. F., & Green, B. (2016). Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS. American Journal of Translational Research, 8(1), 154-165. http://www.ajtr.org/files/ajtr0018204.pdf

Vancouver

Nasaruddin ML, Holscher C, Kehoe P, Graham SF, Green B. Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS. American Journal of Translational Research. 2016 Jan 30;8(1):154-165.

Author

Nasaruddin, Muhammad Luqman ; Holscher, Christian ; Kehoe, Patrick ; Graham, Stewart F. ; Green, Brian. / Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS. In: American Journal of Translational Research. 2016 ; Vol. 8, No. 1. pp. 154-165.

Bibtex

@article{3389ee2f0de246d0b1428daba0fd1801,
title = "Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer{\textquoteright}s disease as detected by GC-MS",
abstract = "Disturbed lipid metabolism is a well-established feature of human Alzheimer{\textquoteright}s disease (AD). The present study used gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters (FAMES) to profile all detectable fatty acid (FA) species present in post-mortem neocortical tissue (Brodmann 7 region). Quantitative targeted analysis was undertaken from 29 subjects (n=15 age-matched controls; n=14 late-stage AD). GC-MS analysis of FAMES detected a total of 24 FAs and of these, 20 were fully quantifiable. The results showed significant and wide ranging elevations in AD brain FA concentrations. A total of 9 FAs were elevated in AD with cis-13,16-docosenoic acid increased most (170%; P=0.033). Intriguingly, docosahexanoic acid (DHA; C22:6) concentrations were elevated (47%; P=0.018) which conflicts with the findings of others (unaltered or decreased) in some brain regions after the onset of AD. Furthermore, our results appear to indicate that subject gender influences brain FA levels in AD subjects (but not in age-matched control subjects). Among AD subjects 7 FA species were significantly higher in males than in females. These preliminary findings pinpoint FA disturbances as potentially important in thepathology of AD. Further work is required to determine if such changes are influenced by disease severity or different types of dementia. ",
keywords = "Fatty acid, GC-MS, AD, brain tissues, DHA",
author = "Nasaruddin, {Muhammad Luqman} and Christian Holscher and Patrick Kehoe and Graham, {Stewart F.} and Brian Green",
year = "2016",
month = jan,
day = "30",
language = "English",
volume = "8",
pages = "154--165",
journal = "American Journal of Translational Research",
issn = "1943-8141",
publisher = "e-Century Publishing Corporation",
number = "1",

}

RIS

TY - JOUR

T1 - Wide-ranging alterations in the brain fatty acid complement of subjects with late Alzheimer’s disease as detected by GC-MS

AU - Nasaruddin, Muhammad Luqman

AU - Holscher, Christian

AU - Kehoe, Patrick

AU - Graham, Stewart F.

AU - Green, Brian

PY - 2016/1/30

Y1 - 2016/1/30

N2 - Disturbed lipid metabolism is a well-established feature of human Alzheimer’s disease (AD). The present study used gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters (FAMES) to profile all detectable fatty acid (FA) species present in post-mortem neocortical tissue (Brodmann 7 region). Quantitative targeted analysis was undertaken from 29 subjects (n=15 age-matched controls; n=14 late-stage AD). GC-MS analysis of FAMES detected a total of 24 FAs and of these, 20 were fully quantifiable. The results showed significant and wide ranging elevations in AD brain FA concentrations. A total of 9 FAs were elevated in AD with cis-13,16-docosenoic acid increased most (170%; P=0.033). Intriguingly, docosahexanoic acid (DHA; C22:6) concentrations were elevated (47%; P=0.018) which conflicts with the findings of others (unaltered or decreased) in some brain regions after the onset of AD. Furthermore, our results appear to indicate that subject gender influences brain FA levels in AD subjects (but not in age-matched control subjects). Among AD subjects 7 FA species were significantly higher in males than in females. These preliminary findings pinpoint FA disturbances as potentially important in thepathology of AD. Further work is required to determine if such changes are influenced by disease severity or different types of dementia.

AB - Disturbed lipid metabolism is a well-established feature of human Alzheimer’s disease (AD). The present study used gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters (FAMES) to profile all detectable fatty acid (FA) species present in post-mortem neocortical tissue (Brodmann 7 region). Quantitative targeted analysis was undertaken from 29 subjects (n=15 age-matched controls; n=14 late-stage AD). GC-MS analysis of FAMES detected a total of 24 FAs and of these, 20 were fully quantifiable. The results showed significant and wide ranging elevations in AD brain FA concentrations. A total of 9 FAs were elevated in AD with cis-13,16-docosenoic acid increased most (170%; P=0.033). Intriguingly, docosahexanoic acid (DHA; C22:6) concentrations were elevated (47%; P=0.018) which conflicts with the findings of others (unaltered or decreased) in some brain regions after the onset of AD. Furthermore, our results appear to indicate that subject gender influences brain FA levels in AD subjects (but not in age-matched control subjects). Among AD subjects 7 FA species were significantly higher in males than in females. These preliminary findings pinpoint FA disturbances as potentially important in thepathology of AD. Further work is required to determine if such changes are influenced by disease severity or different types of dementia.

KW - Fatty acid

KW - GC-MS

KW - AD

KW - brain tissues

KW - DHA

M3 - Journal article

VL - 8

SP - 154

EP - 165

JO - American Journal of Translational Research

JF - American Journal of Translational Research

SN - 1943-8141

IS - 1

ER -