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Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus

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Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus. / Molloy, Jennifer C.; Sinkins, Steven Paul.
In: Viruses, Vol. 7, No. 8, 13.08.2015, p. 4624-4639.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Molloy, JC & Sinkins, SP 2015, 'Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus', Viruses, vol. 7, no. 8, pp. 4624-4639. https://doi.org/10.3390/v7082836

APA

Molloy, J. C., & Sinkins, S. P. (2015). Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus. Viruses, 7(8), 4624-4639. https://doi.org/10.3390/v7082836

Vancouver

Molloy JC, Sinkins SP. Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus. Viruses. 2015 Aug 13;7(8):4624-4639. doi: 10.3390/v7082836

Author

Molloy, Jennifer C. ; Sinkins, Steven Paul. / Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus. In: Viruses. 2015 ; Vol. 7, No. 8. pp. 4624-4639.

Bibtex

@article{950c2eb90d3e4b24b1142841ae47a0b7,
title = "Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus",
abstract = "Aedes albopictus is a major vector of dengue (DENV) and chikungunya (CHIKV) viruses, causing millions of infections annually. It naturally carries, at high frequency, the intracellular inherited bacterial endosymbiont Wolbachia strains wAlbA and wAlbB; transinfection with the higher-density Wolbachia strain wMel from Drosophila melanogaster led to transmission blocking of both arboviruses. The hypothesis that reactive oxygen species (ROS)-induced immune activation plays a role in arbovirus inhibition in this species was examined. In contrast to previous observations in Ae. aegypti, elevation of ROS levels was not observed in either cell lines or mosquito lines carrying the wild-type Wolbachia or higher-density Drosophila Wolbachia strains. There was also no upregulation of genes controlling innate immune pathways or with antioxidant/ROS-producing functions. These data suggest that ROS-mediated immune activation is not an important component of the viral transmission-blocking phenotype in this species.",
keywords = "mosquito, Aedes, arbovirus, dengue, chikungunya, ROS",
author = "Molloy, {Jennifer C.} and Sinkins, {Steven Paul}",
year = "2015",
month = aug,
day = "13",
doi = "10.3390/v7082836",
language = "English",
volume = "7",
pages = "4624--4639",
journal = "Viruses",
issn = "1999-4915",
publisher = "MDPI AG",
number = "8",

}

RIS

TY - JOUR

T1 - Wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus

AU - Molloy, Jennifer C.

AU - Sinkins, Steven Paul

PY - 2015/8/13

Y1 - 2015/8/13

N2 - Aedes albopictus is a major vector of dengue (DENV) and chikungunya (CHIKV) viruses, causing millions of infections annually. It naturally carries, at high frequency, the intracellular inherited bacterial endosymbiont Wolbachia strains wAlbA and wAlbB; transinfection with the higher-density Wolbachia strain wMel from Drosophila melanogaster led to transmission blocking of both arboviruses. The hypothesis that reactive oxygen species (ROS)-induced immune activation plays a role in arbovirus inhibition in this species was examined. In contrast to previous observations in Ae. aegypti, elevation of ROS levels was not observed in either cell lines or mosquito lines carrying the wild-type Wolbachia or higher-density Drosophila Wolbachia strains. There was also no upregulation of genes controlling innate immune pathways or with antioxidant/ROS-producing functions. These data suggest that ROS-mediated immune activation is not an important component of the viral transmission-blocking phenotype in this species.

AB - Aedes albopictus is a major vector of dengue (DENV) and chikungunya (CHIKV) viruses, causing millions of infections annually. It naturally carries, at high frequency, the intracellular inherited bacterial endosymbiont Wolbachia strains wAlbA and wAlbB; transinfection with the higher-density Wolbachia strain wMel from Drosophila melanogaster led to transmission blocking of both arboviruses. The hypothesis that reactive oxygen species (ROS)-induced immune activation plays a role in arbovirus inhibition in this species was examined. In contrast to previous observations in Ae. aegypti, elevation of ROS levels was not observed in either cell lines or mosquito lines carrying the wild-type Wolbachia or higher-density Drosophila Wolbachia strains. There was also no upregulation of genes controlling innate immune pathways or with antioxidant/ROS-producing functions. These data suggest that ROS-mediated immune activation is not an important component of the viral transmission-blocking phenotype in this species.

KW - mosquito

KW - Aedes

KW - arbovirus

KW - dengue

KW - chikungunya

KW - ROS

U2 - 10.3390/v7082836

DO - 10.3390/v7082836

M3 - Journal article

VL - 7

SP - 4624

EP - 4639

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 8

ER -