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XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus.

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XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus. / Jones, Rhiannon E.; Chapman, J. Ross; Puligilla, Chandrakala; Murray, Johanne M.; Car, Antony M.; Ford, Christopher C.; Lindsay, Howard D.

In: Molecular Biology of the Cell, Vol. 14, No. 9, 2003, p. 3898-3910.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jones, RE, Chapman, JR, Puligilla, C, Murray, JM, Car, AM, Ford, CC & Lindsay, HD 2003, 'XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus.', Molecular Biology of the Cell, vol. 14, no. 9, pp. 3898-3910. https://doi.org/10.1091/mbc.E03-03-0138

APA

Jones, R. E., Chapman, J. R., Puligilla, C., Murray, J. M., Car, A. M., Ford, C. C., & Lindsay, H. D. (2003). XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus. Molecular Biology of the Cell, 14(9), 3898-3910. https://doi.org/10.1091/mbc.E03-03-0138

Vancouver

Jones RE, Chapman JR, Puligilla C, Murray JM, Car AM, Ford CC et al. XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus. Molecular Biology of the Cell. 2003;14(9):3898-3910. https://doi.org/10.1091/mbc.E03-03-0138

Author

Jones, Rhiannon E. ; Chapman, J. Ross ; Puligilla, Chandrakala ; Murray, Johanne M. ; Car, Antony M. ; Ford, Christopher C. ; Lindsay, Howard D. / XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus. In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 9. pp. 3898-3910.

Bibtex

@article{0ece8f8e7f834e92a066ff858b1209c6,
title = "XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus.",
abstract = "The DNA damage/replication checkpoints act by sensing the presence of damaged DNA or stalled replication forks and initiate signaling pathways that arrest cell cycle progression. Here we report the cloning and characterization of Xenopus orthologues of the RFCand PCNA-related checkpoint proteins. XRad17 shares regions of homology with the five subunits of Replication factor C. XRad9, XRad1, and XHus1 (components of the 9-1-1 complex) all show homology to the DNA polymerase processivity factor PCNA. We demonstrate that these proteins associate with chromatin and are phosphorylated when replication is inhibited by aphidicolin. Phosphorylation of X9-1-1 is caffeine sensitive, but the chromatin association of XRad17 and the X9-1-1 complex after replication block is unaffected by caffeine. This suggests that the X9-1-1 complex can associate with chromatin independently of XAtm/XAtr activity. We further demonstrate that XRad17 is essential for the chromatin binding and checkpoint-dependent phosphorylation of X9-1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin. XRad17 is not, however, required for the activation of XCds1 in response to dsDNA ends",
author = "Jones, {Rhiannon E.} and Chapman, {J. Ross} and Chandrakala Puligilla and Murray, {Johanne M.} and Car, {Antony M.} and Ford, {Christopher C.} and Lindsay, {Howard D.}",
year = "2003",
doi = "10.1091/mbc.E03-03-0138",
language = "English",
volume = "14",
pages = "3898--3910",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "9",

}

RIS

TY - JOUR

T1 - XRad17 Is Required for the Activation of XChk1 But Not XCds1 during Checkpoint Signaling in Xenopus.

AU - Jones, Rhiannon E.

AU - Chapman, J. Ross

AU - Puligilla, Chandrakala

AU - Murray, Johanne M.

AU - Car, Antony M.

AU - Ford, Christopher C.

AU - Lindsay, Howard D.

PY - 2003

Y1 - 2003

N2 - The DNA damage/replication checkpoints act by sensing the presence of damaged DNA or stalled replication forks and initiate signaling pathways that arrest cell cycle progression. Here we report the cloning and characterization of Xenopus orthologues of the RFCand PCNA-related checkpoint proteins. XRad17 shares regions of homology with the five subunits of Replication factor C. XRad9, XRad1, and XHus1 (components of the 9-1-1 complex) all show homology to the DNA polymerase processivity factor PCNA. We demonstrate that these proteins associate with chromatin and are phosphorylated when replication is inhibited by aphidicolin. Phosphorylation of X9-1-1 is caffeine sensitive, but the chromatin association of XRad17 and the X9-1-1 complex after replication block is unaffected by caffeine. This suggests that the X9-1-1 complex can associate with chromatin independently of XAtm/XAtr activity. We further demonstrate that XRad17 is essential for the chromatin binding and checkpoint-dependent phosphorylation of X9-1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin. XRad17 is not, however, required for the activation of XCds1 in response to dsDNA ends

AB - The DNA damage/replication checkpoints act by sensing the presence of damaged DNA or stalled replication forks and initiate signaling pathways that arrest cell cycle progression. Here we report the cloning and characterization of Xenopus orthologues of the RFCand PCNA-related checkpoint proteins. XRad17 shares regions of homology with the five subunits of Replication factor C. XRad9, XRad1, and XHus1 (components of the 9-1-1 complex) all show homology to the DNA polymerase processivity factor PCNA. We demonstrate that these proteins associate with chromatin and are phosphorylated when replication is inhibited by aphidicolin. Phosphorylation of X9-1-1 is caffeine sensitive, but the chromatin association of XRad17 and the X9-1-1 complex after replication block is unaffected by caffeine. This suggests that the X9-1-1 complex can associate with chromatin independently of XAtm/XAtr activity. We further demonstrate that XRad17 is essential for the chromatin binding and checkpoint-dependent phosphorylation of X9-1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin. XRad17 is not, however, required for the activation of XCds1 in response to dsDNA ends

U2 - 10.1091/mbc.E03-03-0138

DO - 10.1091/mbc.E03-03-0138

M3 - Journal article

VL - 14

SP - 3898

EP - 3910

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 9

ER -