Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Bimodal responses of cells to trace elements
T2 - insights into their mechanism of action using a biospectroscopy approach
AU - Llabjani, Valon
AU - Hoti - Llabjani, Valmira
AU - Mohammad Pouran, Hamid
AU - Martin, Francis Luke
AU - Zhang, Hao
PY - 2014/10
Y1 - 2014/10
N2 - Understanding how organisms respond to trace elements is important because some are essential for normal bodily homeostasis, but can additionally be toxic at high concentrations. The inflection point for many of these elements is unknown and requires sensitive techniques capable of detecting subtle cellular changes as well as cytotoxic alterations. In this study, we treated human cells with arsenic (As), copper or selenium (Se) in a dose–response manner and used attenuated total reflection Fourier-transform infrared (ATR-FTIR) microspectroscopy combined with computational analysis to examine cellular alterations. Cell cultures were treated with Asv, Cu2+ or Seiv at concentrations ranging from 0.001 mg L−1 to 1000 mg L−1 and their effects were spectrochemically determined. Results show that Asv and Cu2+ induce bimodal dose–response effects on cells; this is in line with hormesis-driven responses. Lipids and proteins seem to be the main cell targets for all the elements tested; however, each compound produced a unique fingerprint of effect. Spectral biomarkers indicate that all test agents generate reactive oxygen species (ROS), which could either stimulate repair mechanisms or induce damage in cells.
AB - Understanding how organisms respond to trace elements is important because some are essential for normal bodily homeostasis, but can additionally be toxic at high concentrations. The inflection point for many of these elements is unknown and requires sensitive techniques capable of detecting subtle cellular changes as well as cytotoxic alterations. In this study, we treated human cells with arsenic (As), copper or selenium (Se) in a dose–response manner and used attenuated total reflection Fourier-transform infrared (ATR-FTIR) microspectroscopy combined with computational analysis to examine cellular alterations. Cell cultures were treated with Asv, Cu2+ or Seiv at concentrations ranging from 0.001 mg L−1 to 1000 mg L−1 and their effects were spectrochemically determined. Results show that Asv and Cu2+ induce bimodal dose–response effects on cells; this is in line with hormesis-driven responses. Lipids and proteins seem to be the main cell targets for all the elements tested; however, each compound produced a unique fingerprint of effect. Spectral biomarkers indicate that all test agents generate reactive oxygen species (ROS), which could either stimulate repair mechanisms or induce damage in cells.
KW - ATR-FTIR spectroscopy
KW - Arsenic
KW - Copper
KW - Selenium
KW - Hormesis
KW - Dose-response
U2 - 10.1016/j.chemosphere.2014.03.117
DO - 10.1016/j.chemosphere.2014.03.117
M3 - Journal article
VL - 112
SP - 377
EP - 384
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
ER -