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Extraction of Rubisco to Determine Catalytic Constants

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)

Published
Publication date6/07/2018
Host publicationPhotosynthesis: Methods and Protocols
EditorsSarah Covshoff
Place of PublicationNew York, NY, USA
PublisherSpringer
Pages229-238
Number of pages10
Volume1770
ISBN (Electronic)9781493977864
ISBN (Print)9781493977857
Original languageEnglish

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1770
ISSN (Print)1064-3745

Abstract

Rubisco enables net carbon fixation through the carboxylation of RuBP during photosynthesis. Its complex biochemistry and the catalytic diversity found amongst different plants makes characterization of Rubisco properties useful for investigations aimed at improving photosynthetic performance. This chapter reports methods for rapid extraction of soluble proteins to examine Rubisco catalytic properties, and for large scale purification of Rubisco from leaves to measure the specificity of the enzyme towards its gaseous substrates.