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Extraction of Rubisco to Determine Catalytic Constants

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)peer-review

Publication date6/07/2018
Host publicationPhotosynthesis: Methods and Protocols
EditorsSarah Covshoff
Place of PublicationNew York, NY, USA
Number of pages10
ISBN (Electronic)9781493977864
ISBN (Print)9781493977857
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


Rubisco enables net carbon fixation through the carboxylation of RuBP during photosynthesis. Its complex biochemistry and the catalytic diversity found amongst different plants makes characterization of Rubisco properties useful for investigations aimed at improving photosynthetic performance. This chapter reports methods for rapid extraction of soluble proteins to examine Rubisco catalytic properties, and for large scale purification of Rubisco from leaves to measure the specificity of the enzyme towards its gaseous substrates.