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Extraction of Rubisco to Determine Catalytic Constants

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

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Extraction of Rubisco to Determine Catalytic Constants. / Orr, Douglas John; Carmo-Silva, Ana Elizabete.
Photosynthesis: Methods and Protocols. ed. / Sarah Covshoff. Vol. 1770 New York, NY, USA: Springer, 2018. p. 229-238 (Methods in Molecular Biology; Vol. 1770).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Orr, DJ & Carmo-Silva, AE 2018, Extraction of Rubisco to Determine Catalytic Constants. in S Covshoff (ed.), Photosynthesis: Methods and Protocols. vol. 1770, Methods in Molecular Biology, vol. 1770, Springer, New York, NY, USA, pp. 229-238.

APA

Orr, D. J., & Carmo-Silva, A. E. (2018). Extraction of Rubisco to Determine Catalytic Constants. In S. Covshoff (Ed.), Photosynthesis: Methods and Protocols (Vol. 1770, pp. 229-238). (Methods in Molecular Biology; Vol. 1770). Springer.

Vancouver

Orr DJ, Carmo-Silva AE. Extraction of Rubisco to Determine Catalytic Constants. In Covshoff S, editor, Photosynthesis: Methods and Protocols. Vol. 1770. New York, NY, USA: Springer. 2018. p. 229-238. (Methods in Molecular Biology).

Author

Orr, Douglas John ; Carmo-Silva, Ana Elizabete. / Extraction of Rubisco to Determine Catalytic Constants. Photosynthesis: Methods and Protocols. editor / Sarah Covshoff. Vol. 1770 New York, NY, USA : Springer, 2018. pp. 229-238 (Methods in Molecular Biology).

Bibtex

@inbook{1ba82ca5fc1d42fdaabfb2a32f76d9b9,
title = "Extraction of Rubisco to Determine Catalytic Constants",
abstract = "Rubisco enables net carbon fixation through the carboxylation of RuBP during photosynthesis. Its complex biochemistry and the catalytic diversity found amongst different plants makes characterization of Rubisco properties useful for investigations aimed at improving photosynthetic performance. This chapter reports methods for rapid extraction of soluble proteins to examine Rubisco catalytic properties, and for large scale purification of Rubisco from leaves to measure the specificity of the enzyme towards its gaseous substrates.",
author = "Orr, {Douglas John} and Carmo-Silva, {Ana Elizabete}",
year = "2018",
month = jul,
day = "6",
language = "English",
isbn = "9781493977857",
volume = "1770",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "229--238",
editor = "Sarah Covshoff",
booktitle = "Photosynthesis",

}

RIS

TY - CHAP

T1 - Extraction of Rubisco to Determine Catalytic Constants

AU - Orr, Douglas John

AU - Carmo-Silva, Ana Elizabete

PY - 2018/7/6

Y1 - 2018/7/6

N2 - Rubisco enables net carbon fixation through the carboxylation of RuBP during photosynthesis. Its complex biochemistry and the catalytic diversity found amongst different plants makes characterization of Rubisco properties useful for investigations aimed at improving photosynthetic performance. This chapter reports methods for rapid extraction of soluble proteins to examine Rubisco catalytic properties, and for large scale purification of Rubisco from leaves to measure the specificity of the enzyme towards its gaseous substrates.

AB - Rubisco enables net carbon fixation through the carboxylation of RuBP during photosynthesis. Its complex biochemistry and the catalytic diversity found amongst different plants makes characterization of Rubisco properties useful for investigations aimed at improving photosynthetic performance. This chapter reports methods for rapid extraction of soluble proteins to examine Rubisco catalytic properties, and for large scale purification of Rubisco from leaves to measure the specificity of the enzyme towards its gaseous substrates.

M3 - Chapter (peer-reviewed)

SN - 9781493977857

VL - 1770

T3 - Methods in Molecular Biology

SP - 229

EP - 238

BT - Photosynthesis

A2 - Covshoff, Sarah

PB - Springer

CY - New York, NY, USA

ER -