TY - JOUR

T1 - Isolation of clathrin-coated vesicles from tissue culture cells

AU - Borner, Georg H H

AU - Fielding, Andrew B.

N1 - © 2014 Cold Spring Harbor Laboratory Press.

PY - 2014/11/3

Y1 - 2014/11/3

N2 - The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.

AB - The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.

KW - Cell Fractionation

KW - Cells, Cultured

KW - Clathrin-Coated Vesicles

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1101/pdb.top074435

DO - 10.1101/pdb.top074435

M3 - Journal article

C2 - 25368317

VL - 2014

SP - 1136

EP - 1138

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 11

ER -