Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Isolation of clathrin-coated vesicles from tissue culture cells
AU - Borner, Georg H H
AU - Fielding, Andrew B.
N1 - © 2014 Cold Spring Harbor Laboratory Press.
PY - 2014/11/3
Y1 - 2014/11/3
N2 - The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.
AB - The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.
KW - Cell Fractionation
KW - Cells, Cultured
KW - Clathrin-Coated Vesicles
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1101/pdb.top074435
DO - 10.1101/pdb.top074435
M3 - Journal article
C2 - 25368317
VL - 2014
SP - 1136
EP - 1138
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 11
ER -