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Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

Research output: Contribution in Book/Report/ProceedingsChapter (peer-reviewed)

Forthcoming
Publication date22/05/2018
Host publicationPhotosynthesis: Methods and Protocols
EditorsSarah Covshoff
PublisherSpringer Science + Business Media
ISBN (Electronic)9781493977864
ISBN (Print)9781493977857
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1770
ISSN (Print)1064-3745

Abstract

In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.