Final published version
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review
}
TY - CHAP
T1 - Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting
AU - Perdomo Lopez, Alejandro
AU - Sales, Cristina R. G.
AU - Carmo-Silva, Ana Elizabete
PY - 2018/7/6
Y1 - 2018/7/6
N2 - In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.
AB - In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.
U2 - 10.1007/978-1-4939-7786-4_12
DO - 10.1007/978-1-4939-7786-4_12
M3 - Chapter (peer-reviewed)
SN - 9781493977857
SN - 9781493992850
T3 - Methods in Molecular Biology
SP - 215
EP - 227
BT - Photosynthesis
A2 - Covshoff, Sarah
PB - Humana Press
CY - New York
ER -