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Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

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Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting. / Perdomo Lopez, Alejandro; Sales, Cristina R. G.; Carmo-Silva, Ana Elizabete.
Photosynthesis: Methods and Protocols. ed. / Sarah Covshoff. New York: Humana Press, 2018. p. 215-227 (Methods in Molecular Biology; Vol. 1770).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Perdomo Lopez, A, Sales, CRG & Carmo-Silva, AE 2018, Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting. in S Covshoff (ed.), Photosynthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1770, Humana Press, New York, pp. 215-227. https://doi.org/10.1007/978-1-4939-7786-4_12

APA

Perdomo Lopez, A., Sales, C. R. G., & Carmo-Silva, A. E. (2018). Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting. In S. Covshoff (Ed.), Photosynthesis: Methods and Protocols (pp. 215-227). (Methods in Molecular Biology; Vol. 1770). Humana Press. https://doi.org/10.1007/978-1-4939-7786-4_12

Vancouver

Perdomo Lopez A, Sales CRG, Carmo-Silva AE. Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting. In Covshoff S, editor, Photosynthesis: Methods and Protocols. New York: Humana Press. 2018. p. 215-227. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-7786-4_12

Author

Perdomo Lopez, Alejandro ; Sales, Cristina R. G. ; Carmo-Silva, Ana Elizabete. / Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting. Photosynthesis: Methods and Protocols. editor / Sarah Covshoff. New York : Humana Press, 2018. pp. 215-227 (Methods in Molecular Biology).

Bibtex

@inbook{a0c76cbcc9084b47b40d645881b2dab2,
title = "Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting",
abstract = "In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.",
author = "{Perdomo Lopez}, Alejandro and Sales, {Cristina R. G.} and Carmo-Silva, {Ana Elizabete}",
year = "2018",
month = jul,
day = "6",
doi = "10.1007/978-1-4939-7786-4_12",
language = "English",
isbn = "9781493977857",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "215--227",
editor = "Sarah Covshoff",
booktitle = "Photosynthesis",

}

RIS

TY - CHAP

T1 - Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

AU - Perdomo Lopez, Alejandro

AU - Sales, Cristina R. G.

AU - Carmo-Silva, Ana Elizabete

PY - 2018/7/6

Y1 - 2018/7/6

N2 - In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.

AB - In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene editing approaches. We focus on Rubisco and Rubisco activase, a molecular chaperone required to sustain the activity of Rubisco and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.

U2 - 10.1007/978-1-4939-7786-4_12

DO - 10.1007/978-1-4939-7786-4_12

M3 - Chapter (peer-reviewed)

SN - 9781493977857

SN - 9781493992850

T3 - Methods in Molecular Biology

SP - 215

EP - 227

BT - Photosynthesis

A2 - Covshoff, Sarah

PB - Humana Press

CY - New York

ER -