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  • Jackson-Jones Immunity AAM for PURE

    Rights statement: This is the author’s version of a work that was accepted for publication in Immunity. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Immunity, 52, 4, 2020 DOI: 10.1016/j.immuni.2020.03.011

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Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants

Research output: Contribution to journalJournal article

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  • Lucy Jackson-Jones
  • Peter Smith
  • Jordan Portman
  • Marlène Sophie Magalhaes
  • Katie Mylonas
  • Matthieu Vermeren
  • Mark Nixon
  • Beth Henderson
  • Ross Dobie
  • Sonja Vermeren
  • Laura Denby
  • Neil Henderson
  • Damian Mole
  • Cécile Bénézech
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<mark>Journal publication date</mark>14/04/2020
<mark>Journal</mark>Immunity
Issue number4
Volume52
Number of pages16
Pages (from-to)700-715
Publication StatusPublished
<mark>Original language</mark>English

Abstract

The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Immunity. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Immunity, 52, 4, 2020 DOI: 10.1016/j.immuni.2020.03.011