Home > Research > Publications & Outputs > Stromal cells covering omental fat-associated l...

Research

Associated organisational unit

Electronic data

  • Jackson-Jones Immunity AAM for PURE

    Rights statement: This is the author’s version of a work that was accepted for publication in Immunity. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Immunity, 52, 4, 2020 DOI: 10.1016/j.immuni.2020.03.011

    Accepted author manuscript, 75.3 MB, PDF document

    Available under license: CC BY: Creative Commons Attribution 4.0 International License

Links

Text available via DOI:

View graph of relations

Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants. / Jackson-Jones, Lucy; Smith, Peter; Portman, Jordan et al.
In: Immunity, Vol. 52, No. 4, 14.04.2020, p. 700-715.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Jackson-Jones, L, Smith, P, Portman, J, Magalhaes, MS, Mylonas, K, Vermeren, M, Nixon, M, Henderson, B, Dobie, R, Vermeren, S, Denby, L, Henderson, N, Mole, D & Bénézech, C 2020, 'Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants', Immunity, vol. 52, no. 4, pp. 700-715. https://doi.org/10.1016/j.immuni.2020.03.011

APA

Jackson-Jones, L., Smith, P., Portman, J., Magalhaes, M. S., Mylonas, K., Vermeren, M., Nixon, M., Henderson, B., Dobie, R., Vermeren, S., Denby, L., Henderson, N., Mole, D., & Bénézech, C. (2020). Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants. Immunity, 52(4), 700-715. https://doi.org/10.1016/j.immuni.2020.03.011

Vancouver

Jackson-Jones L, Smith P, Portman J, Magalhaes MS, Mylonas K, Vermeren M et al. Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants. Immunity. 2020 Apr 14;52(4):700-715. doi: 10.1016/j.immuni.2020.03.011

Author

Jackson-Jones, Lucy ; Smith, Peter ; Portman, Jordan et al. / Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants. In: Immunity. 2020 ; Vol. 52, No. 4. pp. 700-715.

Bibtex

@article{6362c22f21734fe9b5c7797f90e142f2,
title = "Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants",
abstract = "The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.",
author = "Lucy Jackson-Jones and Peter Smith and Jordan Portman and Magalhaes, {Marl{\`e}ne Sophie} and Katie Mylonas and Matthieu Vermeren and Mark Nixon and Beth Henderson and Ross Dobie and Sonja Vermeren and Laura Denby and Neil Henderson and Damian Mole and C{\'e}cile B{\'e}n{\'e}zech",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Immunity. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Immunity, 52, 4, 2020 DOI: 10.1016/j.immuni.2020.03.011",
year = "2020",
month = apr,
day = "14",
doi = "10.1016/j.immuni.2020.03.011",
language = "English",
volume = "52",
pages = "700--715",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Stromal cells covering omental fat-associated lymphoid clusters trigger the formation of neutrophil aggregates to capture peritoneal contaminants

AU - Jackson-Jones, Lucy

AU - Smith, Peter

AU - Portman, Jordan

AU - Magalhaes, Marlène Sophie

AU - Mylonas, Katie

AU - Vermeren, Matthieu

AU - Nixon, Mark

AU - Henderson, Beth

AU - Dobie, Ross

AU - Vermeren, Sonja

AU - Denby, Laura

AU - Henderson, Neil

AU - Mole, Damian

AU - Bénézech, Cécile

N1 - This is the author’s version of a work that was accepted for publication in Immunity. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Immunity, 52, 4, 2020 DOI: 10.1016/j.immuni.2020.03.011

PY - 2020/4/14

Y1 - 2020/4/14

N2 - The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

AB - The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

U2 - 10.1016/j.immuni.2020.03.011

DO - 10.1016/j.immuni.2020.03.011

M3 - Journal article

VL - 52

SP - 700

EP - 715

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 4

ER -