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Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025 in the human gut - Supplementary Figure

Dataset

  • Xin Qian (Creator)
  • Peijun Tian (Creator)
  • Guopeng Lin (Creator)
  • Xinglong Xu (Creator)
  • Gang Wang (Creator)
  • Hao ZhangNanyang Technol Univ, Nanyang Technological University, Nanyang Technological University & National Institute of Education (NIE) Singapore, Sch Mat Sci & Engn, Univ Chinese Acad Sci, Chinese Academy of Sciences, University of Chinese Academy of Sciences, CAS, Huazhong University of Science and Technology, Shanghai Jiao Tong Univ, Shanghai Jiao Tong University, Sch Mech Engn, Univ Arizona, University of Arizona, Changhai Hospital, McGill Univ, McGill University, McGill Sch Environm, Zhongnan Hospital of Wuhan University, Dalian University of Technology, Chongqing Medical University, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China, Fudan Univ, Fudan University, Sch Informat Sci & Technol, Shanghai Stomatological Hospital, Jiangsu Province Hospital, Hubei Academy of Agricultural Sciences, Zhejiang Univ, Zhejiang University, Inst Informat & Commun Engn, Cornell Univ, Cornell University, Kavli Inst Cornell, Shanghai Children's Medical Center, Guangdong Pharmaceutical University, Northwestern Polytechnical University Xian, Second Affiliated Hospital of Harbin Medical University, Shandong University, Qingdao, Shandong 266237, China, Jinan Univ, Jinan University, Coll Pharm, Guangdong Prov Key Lab Pharmacodynam Constituents, Harvard Univ, Harvard University, Dept Phys, Nanchang University, Jilin University, South China University of Technology, Henan Normal University, Yunnan Academy of Agricultural Sciences, Urology Department The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University Qingdao Shandong China, Guangzhou & Chinese Academy of Sciences, Guangdong University of Petrochemical Technology, First Affiliated Hospital of Harbin Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing Medical University, Jiangnan University, The First Affiliated Hospital, Dalian Medical University, Dalian, China, Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Arthritis Research Canada Vancouver British Columbia Canada; Department of Rehabilitation Medicine, West China Hospital Sichuan University Chengdu Sichuan China; Rehabilitation Medicine Key Laboratory of Sichuan Province, West China Hospital Sichuan University Chengdu Sichuan China, Second Affiliated Hospital of Nanjing Medical University, Ruian People's Hospital, Heilongjiang Provincial Hospital, Ocean University of China, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Univ Victoria, University of Victoria, Dept Phys & Astron, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences (Creator)
  • Wei Chen (Creator)

Description

Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.
Date made available2023
PublisherFuture Science Group

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