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Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025in the human gut - Supplementary Table

Dataset

  • Xin Qian (Creator)
  • Peijun Tian (Creator)
  • Guopeng Lin (Creator)
  • Xinglong Xu (Creator)
  • Gang Wang (Creator)
  • Hao ZhangJilin University, Ruian People's Hospital, Urology Department The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University Qingdao Shandong China, Sichuan University, Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Chongqing Medical University, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China, Second Affiliated Hospital of Harbin Medical University, Henan Normal University, Heilongjiang Provincial Hospital, Department of Pathology, The First Affiliated Hospital of Hainan Medical University, Haikou, China;, University of Chinese Academy of Sciences, McGill University, Fudan University, Zhongnan Hospital of Wuhan University, Nanyang Technological University, First Affiliated Hospital of Harbin Medical University, Hubei Academy of Agricultural Sciences, Second Affiliated Hospital of Nanjing Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College, Huazhong University of Science and Technology, Nanchang University, Shanghai Children's Medical Center, Ocean University of China, Northwestern Polytechnical University Xian, University of Victoria, Nanjing Agricultural University, Jiangnan University, South China University of Technology, Nanjing Medical University, Guangdong Pharmaceutical University, Shanghai Stomatological Hospital, Zhejiang University, University of Arizona, Shanghai Jiao Tong University, Chinese Academy of Sciences, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Shandong University, Jiangsu Province Hospital, Jinan University, Cornell University, Yunnan Academy of Agricultural Sciences, Harvard University, Changhai Hospital, Guangdong University of Petrochemical Technology, Dalian University of Technology (Creator)
  • Wei Chen (Creator)

Description

Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.
Date made available2023
PublisherFuture Science Group

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