Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

DOI

https://doi.org/10.6084/m9.figshare.c.6813368

Dataset

Jiajun Yang (Creator)
Peng Shang (Creator)
Bo Zhang (Creator)
Jing Wang (Creator)
Zhenyu Du (Creator)
Shanfeng Wang (Creator)
Jun Xing (Creator)
Hao ZhangJinan University, Shanghai Children's Medical Center, Harvard University, Shanghai Jiao Tong University, University of Victoria, Department of Pathology, The First Affiliated Hospital of Hainan Medical University, Haikou, China;, Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Guangdong Pharmaceutical University, Ruian People's Hospital, Zhongnan Hospital of Wuhan University, South China University of Technology, China agricultural University, Jiangsu Province Hospital, Nanjing Medical University, Jiangnan University, Henan Normal University, Ruijin Hospital, University of Arizona, Guangdong University of Petrochemical Technology, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China, Yunnan Academy of Agricultural Sciences, Nanchang University, Hunan Agricultural University, Wenzhou Medical University, Hubei Academy of Agricultural Sciences, Beijing Anzhen Hospital, McGill University, Northwestern Polytechnical University Xian, Nanjing Agricultural University, Sichuan University, Second Affiliated Hospital of Nanjing Medical University, Jilin University, Changhai Hospital, University of Chinese Academy of Sciences, Urology Department The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University Qingdao Shandong China, Chinese Academy of Medical Sciences & Peking Union Medical College, Chongqing Medical University, Shanghai Stomatological Hospital, Nanyang Technological University, Second Affiliated Hospital of Harbin Medical University, First Affiliated Hospital of Harbin Medical University, Ocean University of China, Dalian University of Technology, Heilongjiang Provincial Hospital, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Shandong University, Zhejiang University, Pudong Medical Center, Huazhong University of Science and Technology, Cornell University, Fudan University, Chinese Academy of Sciences (Creator)

Description

Abstract Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. Results The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. Conclusion The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius.

Date made available	2023
Publisher	Figshare

Contact person

rdm@lancaster.ac.uk

Research

DOI