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Mechanisms underlying the role of endoplasmic reticulum stress in the placental injury and fetal growth restriction in an ovine gestation model

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  • Hao ZhangSouth China University of Technology, Hunan Agricultural University, University of Arizona, Zhongnan Hospital of Wuhan University, Harvard University, Ruian People's Hospital, Hubei Academy of Agricultural Sciences, University of Victoria, Changhai Hospital, Pudong Medical Center, Jiangsu Province Hospital, Sichuan University, Chinese Academy of Medical Sciences & Peking Union Medical College, Yangzhou University, Yunnan Academy of Agricultural Sciences, Zhejiang University, Urology Department The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University Qingdao Shandong China, Fudan University, Shandong University, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Jiangsu University of Science and Technology, Second Affiliated Hospital of Harbin Medical University, Shanghai Stomatological Hospital, Jiangnan University, Northwestern Polytechnical University Xian, Beijing Anzhen Hospital, Jinan University, Department of Pathology, The First Affiliated Hospital of Hainan Medical University, Haikou, China;, Cornell University, Henan Normal University, Nanjing Medical University, Shanghai Children's Medical Center, Chongqing Medical University, Jilin University, Heilongjiang Provincial Hospital, Guangdong University of Petrochemical Technology, Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Wenzhou Medical University, Nanyang Technological University, University of Chinese Academy of Sciences, Huazhong University of Science and Technology, Ocean University of China, Second Affiliated Hospital of Nanjing Medical University, Nanjing Agricultural University, Chinese Academy of Sciences, China agricultural University, Nanchang University, McGill University, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China, Shanghai Jiao Tong University, Dalian University of Technology, Ruijin Hospital, Guangdong Pharmaceutical University, First Affiliated Hospital of Harbin Medical University (Creator)
  • Xia Zha (Creator)
  • Yi Zheng (Creator)
  • Xiaoyun Liu (Creator)
  • Mabrouk Elsabagh (Creator)
  • Hongrong Wang (Creator)
  • Honghua Jiang (Creator)
  • Mengzhi Wang (Creator)

Description

Abstract Background Exposure to bisphenol A (BPA), an environmental pollutant known for its endocrine-disrupting properties, during gestation has been reported to increase the risk of fetal growth restriction (FGR) in an ovine model of pregnancy. We hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta, oxidative stress, inflammatory responses, autophagy and endoplasmic reticulum stress (ERS). However, precise mechanisms underlying the BPA-induced placental dysfunction, and subsequently, FGR, as well as the potential involvement of placental ERS in these complications, remain to be investigated. Methods In vivo experiment, 16 twin-pregnant (from d 40 to 130 of gestation) Hu ewes were randomly distributed into two groups (8 ewes each). One group served as a control and received corn oil once a day, whereas the other group received BPA (5 mg/kg/d as a subcutaneous injection). In vitro study, ovine trophoblast cells (OTCs) were exposed to 4 treatments, 6 replicates each. The OTCs were treated with 400 μmol/L BPA, 400 μmol/L BPA + 0.5 μg/mL tunicamycin (Tm; ERS activator), 400 μmol/L BPA + 1 μmol/L 4-phenyl butyric acid (4-PBA; ERS antagonist) and DMEM/F12 complete medium (control), for 24 h. Results In vivo experiments, pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency, progesterone (P4) level and fetal weight, and an increase in placental estrogen (E2) level, together with barrier dysfunctions, OS, inflammatory responses, autophagy and ERS in type A cotyledons. In vitro experiment, the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy, ERS, pro-apoptosis and inflammatory response, and a decrease in the P4 level and the related protein and gene expressions of antioxidant, anti-apoptosis and barrier function. Moreover, treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine (the increased E2 level and decreased P4 level), OS, inflammatory responses, autophagy, and ERS. However, treating the OTCs with 4-PBA reversed the counteracted effects of Tm mentioned above. Conclusions In general, the results reveal that BPA exposure can cause ERS in the ovine placenta and OTCs, and ERS induction might aggravate BPA-induced dysfunction of the placental barrier and endocrine, OS, inflammatory responses, and autophagy. These data offer novel mechanistic insights into whether ERS is involved in BPA-mediated placental dysfunction and fetal development.
Date made available2023
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