My research group studies the DNA repair mechanisms that safeguard the stability of the genome in mammalian cells. Any perturbation of the physiological progression of the DNA replication machinery causes DNA replication stress, genome-wide or at telomeres, which is counteracted by several DNA repair pathways. Unresolved DNA replication stress leads to gradual accumulation of mutations and DNA damage that facilitate genomic instability, which appears at early stages of disease such as cancer, neurodegenerative disorders and immunodeficiency. By employing a wide range of molecular and cellular biology experimental approaches, with a focus on imaging and DNA fibre assay-based techniques, we are investigating DNA replication stress, RNA:DNA hybrid biology and telomere homeostasis. In depth understanding of the mechanisms that dictate genome stability can be exploited in developing novel targeted therapeutic treatments.