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A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants

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E-pub ahead of print
  • A. Murray
  • D. Palmer
  • D. Bennett
  • V. Dwarampudi
  • J. Pedro de Magalhães
<mark>Journal publication date</mark>9/02/2020
Issue number1-4
Number of pages5
Pages (from-to)29-33
Publication StatusE-pub ahead of print
Early online date9/02/20
<mark>Original language</mark>English


The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.

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Export Date: 20 February 2020