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A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants

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A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants. / Murray, A.; Palmer, D.; Bennett, D.; Dwarampudi, V.; Pedro de Magalhães, J.

In: Fly, Vol. 14, No. 1-4, 09.02.2020, p. 29-33.

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Murray, A. ; Palmer, D. ; Bennett, D. ; Dwarampudi, V. ; Pedro de Magalhães, J. / A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants. In: Fly. 2020 ; Vol. 14, No. 1-4. pp. 29-33.

Bibtex

@article{408bccf6946347e680f2e4c8bd45cfdf,
title = "A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants",
abstract = "The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.",
author = "A. Murray and D. Palmer and D. Bennett and V. Dwarampudi and {Pedro de Magalh{\~a}es}, J.",
note = "Export Date: 20 February 2020",
year = "2020",
month = feb,
day = "9",
doi = "10.1080/19336934.2020.1724051",
language = "English",
volume = "14",
pages = "29--33",
journal = "Fly",
number = "1-4",

}

RIS

TY - JOUR

T1 - A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants

AU - Murray, A.

AU - Palmer, D.

AU - Bennett, D.

AU - Dwarampudi, V.

AU - Pedro de Magalhães, J.

N1 - Export Date: 20 February 2020

PY - 2020/2/9

Y1 - 2020/2/9

N2 - The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.

AB - The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.

U2 - 10.1080/19336934.2020.1724051

DO - 10.1080/19336934.2020.1724051

M3 - Journal article

VL - 14

SP - 29

EP - 33

JO - Fly

JF - Fly

IS - 1-4

ER -