Home > Research > Publications & Outputs > A Non-Destructive, Tuneable Method to Isolate L...

Electronic data

  • microorganisms-09-00680-v2

    Final published version, 4.51 MB, PDF document

    Available under license: CC BY: Creative Commons Attribution 4.0 International License

Links

Text available via DOI:

View graph of relations

A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

Research output: Contribution to journalJournal articlepeer-review

Published
Close
Article number680
<mark>Journal publication date</mark>25/03/2021
<mark>Journal</mark>Microorganisms
Issue number4
Volume9
Number of pages11
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min.