Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - A spectral phenotype of oncogenic human papillomavirus-infected exfoliative cervical cytology distinguishes women based on age
AU - Kerns, Jemma
AU - Cheung, Karen T.
AU - Martin, Cara
AU - O'Leary, John J.
AU - Prendiville, Walter
AU - Martin-Hirsch, Pierre L.
AU - Martin, Francis L.
N1 - Copyright 2010 Elsevier B.V. All rights reserved.
PY - 2010/8/5
Y1 - 2010/8/5
N2 - BACKGROUND: Human papillomavirus (HPV) is a sexually-transmitted infection associated with cervical cancer. Of over 100 HPV types identified, 13 are high-risk oncogenic. In unvaccinated women worldwide, the incidence of cervical cancer from HPV16 and HPV18 will remain. Cervical cytology can be graded from normal (atypia-free) to low-grade to high-grade. Infrared (IR) spectroscopy is a non-destructive technique that allows the acquisition of a biochemical-cell fingerprint based on vibrational states of chemical bonds.METHODS: Exfoliative cervical cytology specimens (n=147) were retrieved, graded by a cytologist and HPV-tested/genotyped using hybrid capture 2 and the Roche HPV Linear Array. Additionally, the spectral signatures of cervical cell lines C33A, HeLa and SiHa were examined. After washing, cellular material was transferred to low-E glass slides and interrogated using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy. Given the complex nature of the dataset consisting of thousands of variables (wavenumbers), we used multivariate analysis for data reduction and information retrieval. Principal component analysis coupled with linear discriminant analysis (PCA-LDA) generated a visual representation of the data (scores plot) and, identification of the wavenumbers and consequent biochemical entities responsible for segregation (loadings plot).RESULTS: Immortalised cell lines were readily distinguishable from each other. It was difficult to segregate categories of cytology associated with HPV infection types. However, in low-grade cytology infected with high-risk oncogenic HPV16 or HPV18, it was possible to segregate women based on whether they were aged 20-29years vs. 30-39years.CONCLUSIONS: Our findings suggest a spectral phenotype in exfoliative cervical cytology associated with transient vs. persistent HPV infection.
AB - BACKGROUND: Human papillomavirus (HPV) is a sexually-transmitted infection associated with cervical cancer. Of over 100 HPV types identified, 13 are high-risk oncogenic. In unvaccinated women worldwide, the incidence of cervical cancer from HPV16 and HPV18 will remain. Cervical cytology can be graded from normal (atypia-free) to low-grade to high-grade. Infrared (IR) spectroscopy is a non-destructive technique that allows the acquisition of a biochemical-cell fingerprint based on vibrational states of chemical bonds.METHODS: Exfoliative cervical cytology specimens (n=147) were retrieved, graded by a cytologist and HPV-tested/genotyped using hybrid capture 2 and the Roche HPV Linear Array. Additionally, the spectral signatures of cervical cell lines C33A, HeLa and SiHa were examined. After washing, cellular material was transferred to low-E glass slides and interrogated using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy. Given the complex nature of the dataset consisting of thousands of variables (wavenumbers), we used multivariate analysis for data reduction and information retrieval. Principal component analysis coupled with linear discriminant analysis (PCA-LDA) generated a visual representation of the data (scores plot) and, identification of the wavenumbers and consequent biochemical entities responsible for segregation (loadings plot).RESULTS: Immortalised cell lines were readily distinguishable from each other. It was difficult to segregate categories of cytology associated with HPV infection types. However, in low-grade cytology infected with high-risk oncogenic HPV16 or HPV18, it was possible to segregate women based on whether they were aged 20-29years vs. 30-39years.CONCLUSIONS: Our findings suggest a spectral phenotype in exfoliative cervical cytology associated with transient vs. persistent HPV infection.
KW - Adult
KW - Age Factors
KW - Aged
KW - Aging
KW - Cell Line, Tumor
KW - Cervix Uteri
KW - Discriminant Analysis
KW - Female
KW - Human papillomavirus 16
KW - Human papillomavirus 18
KW - Humans
KW - Middle Aged
KW - Papillomavirus Infections
KW - Phenotype
KW - Principal Component Analysis
KW - Spectroscopy, Fourier Transform Infrared
KW - Young Adult
U2 - 10.1016/j.cca.2010.03.029
DO - 10.1016/j.cca.2010.03.029
M3 - Journal article
C2 - 20359472
VL - 411
SP - 1027
EP - 1033
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 1873-3492
IS - 15-16
ER -