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A versatile sample injection system for miniaturised isotachophoresis devices

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<mark>Journal publication date</mark>05/2008
<mark>Journal</mark>Microelectronic Engineering
Issue number5-6
Volume85
Number of pages3
Pages (from-to)1440-1442
Publication StatusPublished
Early online date9/02/08
<mark>Original language</mark>English

Abstract

A variable-volume sample injector design is presented and evaluated for performing sample introduction protocols for carrying out miniaturised isotachophoresis (ITP) separations. The microdevice design features a wide bore sample loop channel connected to a narrower bore separation channel via a short injection channel angled at 45°. An additional side arm channel located at the injection point enabled a range of injection strategies, using a gravity-fed system, to be implemented and assessed. A model analyte was used to demonstrate the versatility of the injector design for low and high sample loading regimes suitable for concentrated and dilute sample solutions, respectively.