The aim of this study is to investigate the effect of acute acetaminophen (ACT) ingestion on eight maximal 30-second cycling bouts, interspersed with two-minute rests. Seven recreationally active females (N=7, 22.8±6.7 years of age, with a stature of 163.4±5.8 cm, and body mass of 58.9±5.9 kg) participated in a placebo-controlled, randomized crossover design. Pain perception, heart rate, and power profiles were analyzed using a time x condition repeated measures ANOVA. Blood lactate after the eighth bout and total work done were analyzed using the paired samples t-test. Participants achieved a significantly greater (p=.007, =.725) peak power output during the ACT trials (402.9±73.8 W, 95% CI=334.6–471.1 W) when compared to the placebo (PLA) trials (378.7±64.6 W, 95% CI=319.0–438.4 W). Supplementation of ACT had no effect on mean power output, blood lactate accumulation, pain perception, or total work done after eigth bouts (p>.05). Data presented here suggest females can improve peak power output during repeated Wingate tests with ACT ingestion. It is proposed that the altered afferent feedback may have caused these alterations in power output.