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    Rights statement: This is the author’s version of a work that was accepted for publication in Neurobiology of Aging. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neurobiology of Aging, 101, 2021 DOI: 10.1016/j.neurobiolaging.2020.12.008

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Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus

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Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus. / Frías-Anaya, E.; Gromnicova, R.; Kraev, I.; Rogachevsky, V.; Male, D.K.; Crea, F.; Hawkes, C.A.; Romero, I.A.

In: Neurobiology of Aging, Vol. 101, 31.05.2021, p. 273-284.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Frías-Anaya, E, Gromnicova, R, Kraev, I, Rogachevsky, V, Male, DK, Crea, F, Hawkes, CA & Romero, IA 2021, 'Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus', Neurobiology of Aging, vol. 101, pp. 273-284. https://doi.org/10.1016/j.neurobiolaging.2020.12.008

APA

Frías-Anaya, E., Gromnicova, R., Kraev, I., Rogachevsky, V., Male, D. K., Crea, F., Hawkes, C. A., & Romero, I. A. (2021). Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus. Neurobiology of Aging, 101, 273-284. https://doi.org/10.1016/j.neurobiolaging.2020.12.008

Vancouver

Frías-Anaya E, Gromnicova R, Kraev I, Rogachevsky V, Male DK, Crea F et al. Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus. Neurobiology of Aging. 2021 May 31;101:273-284. https://doi.org/10.1016/j.neurobiolaging.2020.12.008

Author

Frías-Anaya, E. ; Gromnicova, R. ; Kraev, I. ; Rogachevsky, V. ; Male, D.K. ; Crea, F. ; Hawkes, C.A. ; Romero, I.A. / Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus. In: Neurobiology of Aging. 2021 ; Vol. 101. pp. 273-284.

Bibtex

@article{1835c218320742a78ffbf2ef14b92be5,
title = "Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus",
abstract = "Blood-brain barrier (BBB) breakdown occurs in aging and neurodegenerative diseases. Although age-associated alterations have previously been described, most studies focused in male brains; hence, little is known about BBB breakdown in females. This study measured ultrastructural features in the aging female BBB using transmission electron microscopy and 3-dimensional reconstruction of cortical and hippocampal capillaries from 6- and 24-month-old female C57BL/6J mice. Aged cortical capillaries showed more changes than hippocampal capillaries. Specifically, the aged cortex showed thicker basement membrane, higher number and volume of endothelial pseudopods, decreased endothelial mitochondrial number, larger pericyte mitochondria, higher pericyte–endothelial cell contact, and increased tight junction tortuosity compared with young animals. Only increased basement membrane thickness and pericyte mitochondrial volume were observed in the aged hippocampus. Regional comparison revealed significant differences in endothelial pseudopods and tight junctions between the cortex and hippocampus of 24-month-old mice. Therefore, the aging female BBB shows region-specific ultrastructural alterations that may lead to oxidative stress and abnormal capillary blood flow and barrier stability, potentially contributing to cerebrovascular diseases, particularly in postmenopausal women.",
keywords = "Aging, Blood-brain barrier, Capillary ultrastructure, Cerebrovasculature, Female mouse, Transmission electron microscopy",
author = "E. Fr{\'i}as-Anaya and R. Gromnicova and I. Kraev and V. Rogachevsky and D.K. Male and F. Crea and C.A. Hawkes and I.A. Romero",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Neurobiology of Aging. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neurobiology of Aging, 101, 2021 DOI: 10.1016/j.neurobiolaging.2020.12.008",
year = "2021",
month = may,
day = "31",
doi = "10.1016/j.neurobiolaging.2020.12.008",
language = "English",
volume = "101",
pages = "273--284",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Age-related ultrastructural neurovascular changes in the female mouse cortex and hippocampus

AU - Frías-Anaya, E.

AU - Gromnicova, R.

AU - Kraev, I.

AU - Rogachevsky, V.

AU - Male, D.K.

AU - Crea, F.

AU - Hawkes, C.A.

AU - Romero, I.A.

N1 - This is the author’s version of a work that was accepted for publication in Neurobiology of Aging. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neurobiology of Aging, 101, 2021 DOI: 10.1016/j.neurobiolaging.2020.12.008

PY - 2021/5/31

Y1 - 2021/5/31

N2 - Blood-brain barrier (BBB) breakdown occurs in aging and neurodegenerative diseases. Although age-associated alterations have previously been described, most studies focused in male brains; hence, little is known about BBB breakdown in females. This study measured ultrastructural features in the aging female BBB using transmission electron microscopy and 3-dimensional reconstruction of cortical and hippocampal capillaries from 6- and 24-month-old female C57BL/6J mice. Aged cortical capillaries showed more changes than hippocampal capillaries. Specifically, the aged cortex showed thicker basement membrane, higher number and volume of endothelial pseudopods, decreased endothelial mitochondrial number, larger pericyte mitochondria, higher pericyte–endothelial cell contact, and increased tight junction tortuosity compared with young animals. Only increased basement membrane thickness and pericyte mitochondrial volume were observed in the aged hippocampus. Regional comparison revealed significant differences in endothelial pseudopods and tight junctions between the cortex and hippocampus of 24-month-old mice. Therefore, the aging female BBB shows region-specific ultrastructural alterations that may lead to oxidative stress and abnormal capillary blood flow and barrier stability, potentially contributing to cerebrovascular diseases, particularly in postmenopausal women.

AB - Blood-brain barrier (BBB) breakdown occurs in aging and neurodegenerative diseases. Although age-associated alterations have previously been described, most studies focused in male brains; hence, little is known about BBB breakdown in females. This study measured ultrastructural features in the aging female BBB using transmission electron microscopy and 3-dimensional reconstruction of cortical and hippocampal capillaries from 6- and 24-month-old female C57BL/6J mice. Aged cortical capillaries showed more changes than hippocampal capillaries. Specifically, the aged cortex showed thicker basement membrane, higher number and volume of endothelial pseudopods, decreased endothelial mitochondrial number, larger pericyte mitochondria, higher pericyte–endothelial cell contact, and increased tight junction tortuosity compared with young animals. Only increased basement membrane thickness and pericyte mitochondrial volume were observed in the aged hippocampus. Regional comparison revealed significant differences in endothelial pseudopods and tight junctions between the cortex and hippocampus of 24-month-old mice. Therefore, the aging female BBB shows region-specific ultrastructural alterations that may lead to oxidative stress and abnormal capillary blood flow and barrier stability, potentially contributing to cerebrovascular diseases, particularly in postmenopausal women.

KW - Aging

KW - Blood-brain barrier

KW - Capillary ultrastructure

KW - Cerebrovasculature

KW - Female mouse

KW - Transmission electron microscopy

U2 - 10.1016/j.neurobiolaging.2020.12.008

DO - 10.1016/j.neurobiolaging.2020.12.008

M3 - Journal article

VL - 101

SP - 273

EP - 284

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

ER -