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Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo

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Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo. / Cook, Peter C; Jones, Lucy H; Jenkins, Stephen J; Wynn, Thomas A; Allen, Judith E; MacDonald, Andrew S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 25, 19.06.2012, p. 9977-9982.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Cook, PC, Jones, LH, Jenkins, SJ, Wynn, TA, Allen, JE & MacDonald, AS 2012, 'Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 25, pp. 9977-9982. https://doi.org/10.1073/pnas.1121231109

APA

Cook, P. C., Jones, L. H., Jenkins, S. J., Wynn, T. A., Allen, J. E., & MacDonald, A. S. (2012). Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America, 109(25), 9977-9982. https://doi.org/10.1073/pnas.1121231109

Vancouver

Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, MacDonald AS. Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America. 2012 Jun 19;109(25):9977-9982. https://doi.org/10.1073/pnas.1121231109

Author

Cook, Peter C ; Jones, Lucy H ; Jenkins, Stephen J ; Wynn, Thomas A ; Allen, Judith E ; MacDonald, Andrew S. / Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 25. pp. 9977-9982.

Bibtex

@article{cb977e1c4955456ca1635f2dc48aefd2,
title = "Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo",
abstract = "Interleukin-4 is a cytokine widely known for its role in CD4(+) T cell polarization and its ability to alternatively activate macrophage populations. In contrast, the impact of IL-4 on the activation and function of dendritic cells (DCs) is poorly understood. We report here that DCs respond to IL-4 both in vitro and in vivo by expression of multiple alternative activation markers with a different expression pattern to that of macrophages. We further demonstrate a central role for DC IL-4Rα expression in the optimal induction of IFNγ responses in vivo in both Th1 and Th2 settings, through a feedback loop in which IL-4 promotes DC secretion of IL-12. Finally, we reveal a central role for RELMα during T-cell priming, establishing that its expression by DCs is critical for optimal IL-10 and IL-13 promotion in vitro and in vivo. Together, these data highlight the significant impact that IL-4 and RELMα can have on DC activation and function in the context of either bacterial or helminth pathogens.",
keywords = "Animals, CD4-Positive T-Lymphocytes, Cell Polarity, Cytokines, Dendritic Cells, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, In Vitro Techniques, Macrophage Activation, Mice, Mice, Inbred C57BL, Signal Transduction, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Cook, {Peter C} and Jones, {Lucy H} and Jenkins, {Stephen J} and Wynn, {Thomas A} and Allen, {Judith E} and MacDonald, {Andrew S}",
year = "2012",
month = jun,
day = "19",
doi = "10.1073/pnas.1121231109",
language = "English",
volume = "109",
pages = "9977--9982",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "25",

}

RIS

TY - JOUR

T1 - Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo

AU - Cook, Peter C

AU - Jones, Lucy H

AU - Jenkins, Stephen J

AU - Wynn, Thomas A

AU - Allen, Judith E

AU - MacDonald, Andrew S

PY - 2012/6/19

Y1 - 2012/6/19

N2 - Interleukin-4 is a cytokine widely known for its role in CD4(+) T cell polarization and its ability to alternatively activate macrophage populations. In contrast, the impact of IL-4 on the activation and function of dendritic cells (DCs) is poorly understood. We report here that DCs respond to IL-4 both in vitro and in vivo by expression of multiple alternative activation markers with a different expression pattern to that of macrophages. We further demonstrate a central role for DC IL-4Rα expression in the optimal induction of IFNγ responses in vivo in both Th1 and Th2 settings, through a feedback loop in which IL-4 promotes DC secretion of IL-12. Finally, we reveal a central role for RELMα during T-cell priming, establishing that its expression by DCs is critical for optimal IL-10 and IL-13 promotion in vitro and in vivo. Together, these data highlight the significant impact that IL-4 and RELMα can have on DC activation and function in the context of either bacterial or helminth pathogens.

AB - Interleukin-4 is a cytokine widely known for its role in CD4(+) T cell polarization and its ability to alternatively activate macrophage populations. In contrast, the impact of IL-4 on the activation and function of dendritic cells (DCs) is poorly understood. We report here that DCs respond to IL-4 both in vitro and in vivo by expression of multiple alternative activation markers with a different expression pattern to that of macrophages. We further demonstrate a central role for DC IL-4Rα expression in the optimal induction of IFNγ responses in vivo in both Th1 and Th2 settings, through a feedback loop in which IL-4 promotes DC secretion of IL-12. Finally, we reveal a central role for RELMα during T-cell priming, establishing that its expression by DCs is critical for optimal IL-10 and IL-13 promotion in vitro and in vivo. Together, these data highlight the significant impact that IL-4 and RELMα can have on DC activation and function in the context of either bacterial or helminth pathogens.

KW - Animals

KW - CD4-Positive T-Lymphocytes

KW - Cell Polarity

KW - Cytokines

KW - Dendritic Cells

KW - Enzyme-Linked Immunosorbent Assay

KW - Flow Cytometry

KW - Humans

KW - In Vitro Techniques

KW - Macrophage Activation

KW - Mice

KW - Mice, Inbred C57BL

KW - Signal Transduction

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1073/pnas.1121231109

DO - 10.1073/pnas.1121231109

M3 - Journal article

C2 - 22660926

VL - 109

SP - 9977

EP - 9982

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 25

ER -